Alberta Precision Laboratories

--- Query Results ---

Organization:

Alberta Precision Laboratories

Test Name/Synonym:

QuantiFERON-TB Gold Plus
(Synonym: Mycobacterium Tuberculosis, Latent tuberculosis, LTBI, MTB, QFT)

Pathogen:

Mycobacterium tuberculosis

Test Includes:

Serology

Collection Alert:

Outbreak investigation collections are to be arranged directly with the Public Health Laboratories.

See Specimen Collection Requirements for PSC collection locations

Shipping and Handling Alert:

Special Handling requirements – see Processing

LABID (Connect Care):

LAB4299

Primary Container:

QuantiFERON-TB Gold Plus tube, NIL control (grey cap), TB1 antigen (green cap), TB2 antigen (yellow cap), and Mitogen Control (purple cap)

Specimen Collection Requirements:

QuantiFERON-TB Gold Plus tubes, require 1mL of blood.
Please note the black line (red arrow in the below image) present on each tube - this marking represents the acceptable fill volume.
Carefully fill each tube ensuring the blood volume is within the black line.
Blood draws must reach the black line but may not exceed it. Over or underfilling will result in test cancellation.

Repeat into each of the following specific blood collection tubes; NIL control (grey cap), TB1 antigen (green cap), TB2 antigen (yellow cap), and Mitogen Control (purple cap).

Immediately after filling, mix the tubes by shaking them 10 times, just firmly enough to make sure the entire surface of the tube is coated with blood. (Overly vigorous shaking may cause gel disruption and could lead to aberrant results).
Do NOT use any of the QuantiFERON-TB Gold Plus tubes as a discard tube.
Do NOT cover the window with labels.

PSC collection locations:

Calgary and Area	- Bankview PSC - Market Mall PSC - Riverbend PSC - Sheldon Chumir PSC - Sunridge PSC
Edmonton and Area	- Northgate PSC - Heritage PSC - Hys PSC
Urban/Rural	- Red Deer Bower PSC - Red Deer Downtown PSC - Grande Prairie PSC - Fort McMurray Franklin PSC - Lethbridge Centre Village Mall PSC - Lethbridge Highlands PSC - Medicine Hat PSC - Brooks PSC

Test Resources:

QuantiFERON TB Testing 21996

Processing:

If time from sample collection to arrival at Public Health Lab North is	Then
Less than 16 hours	Do not centrifuge Do not refrigerate or freeze Transport with room temperature samples
Greater than 16 hours	Incubate tubes in an upright position at 37℃ for 16-24hrs After incubation, centrifuge tubes for 15 minutes at 3000 RCF(g) After centrifugation, maintain tubes at 4℃ Transport with refrigerated samples If centrifugation is not possible, the tubes can be stored between 4℃ and 27℃ for up to 4 days to allow for transport as long as the incubation step is completed Ensure requisition includes the following information: Specimen#: Set up by: Water bath temp: Incubation start time: Incubation end time: Total incubation time:

Transportation:

Ship as Category B

Refer to https://tc.canada.ca/en/corporate-services/acts-regulations/list-regulations/transportation-dangerous-goods-regulations
APL personnel - refer to HSM14 Transport of Dangerous Goods (TDG) Program

Testing Area:

Virology

Performing Site:

Provincial Laboratory for Public Health (Calgary and Edmonton)

Additional Test Information:

TB Services – in an outbreak situation consult Public Health Lab (ProvLab) Microbiologist On-Call

Last Updated On:

Friday, July 4, 2025

Date of Last Review:

Jan 14 2025 12:00AM