Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

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Test Name/Synonym:

Beta-Hydroxybutyrate (Metabolic Disorders) (Synonyms: Ketone bodies, Quantitative Ketones)

Clinical Indications:

Ketosis is a common feature in acutely ill patients and in some subjects can result in severe life threatening metabolic acidosis. The presence and degree of ketosis can be determined by measuring blood levels of 3-hydroxybutyrate (or D-3-hydroxybutyrate).

This test is for evaluating patients suspected of having an inborn error of metabolism affecting energy production (e.g., hepatic glycogen storage disease) or utilization (e.g., fatty acid oxidation disorders). This test can also be used to monitor adherence of patients treated with the ketogenic diet. This test may also help in the evaluation of diabetes, as well as other conditions leading to or resulting from carbohydrate deprivation, reduced carbohydrate utilization, ketoacidosis, alkalosis or altered cellular redox state.

Test Includes:

Metabolite reported: D-3-hydroxybutyrate

Collection Alert:

Time since last meal should always be indicated for interpretation for planned assessments of fasted (i.e., equal to or greater than 8 hours) or random (i.e., postprandial, less than 8 hours) collections.

LABID (Connect Care):

LAB2336

Test Code:

CALGARY ZONE	BGLBL; IN BGL TEST NAME FIELD ENTER B HYDROXY BUTYR
SOUTH ZONE, MEDICINE HAT	BHRDRO
OTHER ZONES	NOT SPECIFIED

Specimen Type:

Blood

Primary Container:

Green top – Sodium heparin (no gel)

Alternate Containers:

Green top – Lithium heparin (no gel)
Red top – Non-additive (no gel)

Minimum Collection Volume:

0.5 mL plasma or serum

Specimen Collection Requirements:

Collect one Sodium Heparin (or alternate) tube.

Indicate length of fast on the requisition (with collection time).
Indicate if patient is on ketogenic diet and whether sample is fed or fasted.

Test Resources:

Submit request using the applicable requisition or Connect Care online order entry:

Biochemical Genetics Laboratory Requisition

Please ensure all required information, including patient's clinical history/indication, is provided on the requisition.

Stability and Storage:

Frozen: plasma or serum aliquot

Processing:

For ALL collection locations except for Alberta Children’s Hospital (ACH):
1. Centrifuge. If red top tube is collected, allow to fully clot prior to centrifugation.
2. Transfer plasma or serum to aliquot tube.
3. Write "NaHep", "LiHep", or "Red Top" on the aliquot tube label.
4. Freeze aliquot tube.

If collected at ACH outpatient Lab:

The primary tube can be put on ice (ensure not to freeze sample) or refrigerated for routine pick-ups between 08:30 to 14:30, Monday through Friday (excluding holidays).
If specimen is collected after 14:30, process sample as described above.

Transportation:

Transport frozen specimens on dry ice.
Samples must not thaw during transport.

Method:

Spectrophotometric

Method and Interpretation of Results:

Beta-hydroxybutyrate is detected spectrophotometrically using a commercially available kit.

Routine Turn Around Time:

7 days

Testing Schedule:

Weekly

Reference Interval:

Provided on test report, based on patient age and prandial state.

Testing Area:

Biochemical Genetics

Performing Site:

Alberta Children’s Hospital

Additional Test Information:

For priority turn-around contact Biochemical Genetics South Laboratory.

Contact Comments:

Biochemical Genetics 403-955-7379

Last Updated On:

Monday, October 24, 2022

Date of Last Review:

Aug 1 2022 12:00AM