Alberta Precision Laboratories

--- Query Results ---

Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

BCL-1 Translocation (Synonym: t(11;14), Cyclin D1 Translocation)

Clinical Indications:

For the investigation of mantle cell lymphoma.

Test Includes:

Gene: CCND1

Collection Alert:

Unopened dedicated tubes are required for Molecular Pathology testing.

LABID (Connect Care):

LAB4841

Test Code:

OTHER ZONES

NOT SPECIFIED

Specimen Type:

Paraffin-Embedded Tissue, Blood, Bone Marrow, Tissue

Specimen Source:

Indicate a specimen source

Primary Container:

Paraffin Embedded Tissue

Alternate Containers:

Lavender top EDTA
Yellow top ACD (Acid Citrate Dextrose) - B
Bone marrow media
Tissue in flow media

Minimum Collection Volume:

See Specimen Collection Requirements

Specimen Collection Requirements:

Paraffin-Embedded Tissue:

Can be submitted as whole block, precut scrolls, or as blank unstained sections on coated slides.
Representative H&E to accompany the specimen.

Blood:

A minimum of 1.0 mL blood specimen, collected in an EDTA or ACD tube.

Bone Marrow:

A minimum of 2.0 mL bone marrow specimen from Flow Cytometry supplied in Bone Marrow Media (BMM).

Tumor or Tissue (fresh):

A minimum of 10 mg in tissue in flow media.

Test Resources:

Submit request using the requisition or Connect Care online order entry:

Molecular Pathology Requisition

Please ensure all required information, including patient's clinical history/indication, is provided on the requisition.

Stability and Storage:

Room temperature: paraffin-embedded tissue blocks and slides
Refrigerated up to 3 days: blood, bone marrow, fresh tumor or tissue

Processing:

Do not centrifuge.
Do not aliquot.
Do not freeze.
Keep specimens at appropriate storage temperature until ready to transport.

Transportation:

Transport all specimen types at room temperature.
Specimens must not freeze in transit.

Transport to Pathology Department at Foothills Medical Center.

Method:

PCR followed by agarose gel detection

Method and Interpretation of Results:

DNA is extracted and PCR amplified using primers targeting the major translocation cluster (MTC) of the Bcl-1 locus and the immunoglobulin heavy chain (IgH) gene joining region (JH). Samples are run in parallel with positive, negative, and no template controls.

Some breakpoints (as much as 50%) occur at distant loci and will not be identified by this particular test.

Samples producing a PCR product in the valid size range are considered positive for the Bcl-1 translocation.

Routine Turn Around Time:

10 days

Testing Schedule:

Weekly

Testing Area:

Molecular Pathology

Performing Site:

Foothills Medical Center

Contact Comments:

Molecular Pathology South 403-220-4240

Last Updated On:

Monday, June 22, 2020

Date of Last Review:

Aug 1 2022 12:00AM