Alberta Precision Laboratories

--- Query Results ---

Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

BRAF/KRAS/NRAS Analysis, MassArray (Synonyms: BRAF, KRAS, NRAS, EGFR, PIK3CA, colon carcinoma, colon)

Clinical Indications:

For investigation of colon cancer.

Test Includes:

Molecular Pathology North	Molecular Pathology South (FMC)
BRAF, KRAS, NRAS	BRAF, KRAS, NRAS, EGFR, PIK3CA

LABID (Connect Care):

LAB4845

Specimen Type:

Paraffin-Embedded Tissue

Specimen Source:

Indicate specimen source.

Primary Container:

See Specimen Collection Requirements

Specimen Collection Requirements:

Molecular Pathology North	Molecular Pathology South (FMC)
For Paraffin-Embedded Tissue: A formalin-fixed, paraffin-embedded tissue block is preferred. Alternatively, one slide stained with Hematoxylin & Eosin and five 10-micron non-baked, unstained slides can be submitted. Please provide a copy of the corresponding pathology report.	For Paraffin-Embedded Tissue: Can be submitted as whole block, precut scrolls, or as blank unstained sections on coated slides. Representative H&E to accompany the specimen.

Test Resources:

Submit request using the applicable requisition or Connect Care online order entry:

Molecular Pathology North		Molecular Pathology South (FMC)
	Molecular Pathology Requisition		Molecular Pathology Requisition

Please ensure all required information, including patient's clinical history/indication, is provided on the requisition.

Stability and Storage:

Room temperature: paraffin-embedded tissue blocks and slides.

Transportation:

Molecular Pathology North	Molecular Pathology South (FMC)
Transport at room temperature to Molecular Pathology at University of Alberta Hospital.	Transport at room temperature to Pathology Department at Foothills Medical Center.

Method:

Molecular Pathology North
PCR followed by single base extension and mass spectrometry

Molecular Pathology South (FMC)
PCR followed by single base extension and mass spectrometry

Method and Interpretation of Results:

Molecular Pathology North
DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue was performed using the Thermo Fisher MagMAX FFPE DNA/RNA Ultra Kit on the KingFisher Apex purification system.

Testing was performed using the Agena iPLEX HS Colon panel and analyzed using an Agena MassArray matrix assisted laser desorption-ionization time-of-flight mass spectrophotometer (MALDI-TOF MS).

This multiplex assay is capable of detecting the following mutations: BRAF G469E, D594G, V600E; EGFR S492R, KRAS mutation in codons 12, 13, 59, 61, 146, and K117N, K117R; NRAS mutations in codons 12, 13, 59, 61, 146, and K117N, K117R; PIK3CA E542K, E545K, H1047R, H1047L. BRAF V600K mutations will be reported as V600E by this assay.

The estimated sensitivity of this assay is 5%.

Molecular Pathology South (FMC)
Analysis for BRAF, KRAS, NRAS, EGFR and PIK3CA mutations is performed after PCR amplification using a single base extension technique (Agena iPLEX Colon HS assay) and mass spectrometry on the MassArray instrument. This multiplexed assay is capable of detecting BRAF mutations G469E, D594G, and V600E, KRAS/NRAS mutations at codons 12, 13, 59, 61, 117, and 146, and PIK3CA mutaions at codons 542, 545 and 1047. BRAF V600K mutations will be reported as V600E by this assay.

The estimated sensitivity of this assay is 5%.

Routine Turn Around Time:

Molecular Pathology North	Molecular Pathology South (FMC)
2 weeks	10 days

Testing Schedule:

Weekly

Testing Area:

Molecular Pathology

Performing Site:

University of Alberta Hospital or Foothills Medical Center

Contact Comments:

Molecular Pathology North	Molecular Pathology South (FMC)
780-407-6648	403-220-4240

Last Updated On:

Thursday, February 8, 2024

Date of Last Review:

Feb 7 2024 12:00AM