Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

Amino Acids Quantitation, CSF

Clinical Indications:

This test is for the investigation of disorders of amino acid metabolism with specific neurologic presentations (e.g., nonketotic hyperglycinemia, serine biosynthesis disorders, GABA transaminase deficiency).

Test Includes:

Metabolites reported from both BCG North and BCG South:

alanine	arginine	aspartic acid
glutamic acid	glutamine	glycine
histidine	isoleucine	leucine
lysine	methionine	ornithine
phenylalanine	serine	taurine
threonine	tyrosine	valine

Additional metabolites reported from BCG South:

1-methylhistidine	3-methylhistidine	alpha-aminoadipic acid
alpha-aminobutyric acid	anserine	asparagine
beta-alanine	beta-aminoisobutyric acid	carnosine
citrulline	cystathionine	cystine
ethanolamine	gamma-aminobutyric acid	homocystine
hydroxylysine	hydroxyproline	phosphoethanolamine
phosphoserine	proline	sarcosine
tryptophan

Ordering Alert:

This test is considered specialty testing; this test will be cancelled if sufficient clinical justification is not provided.

Amino Acid Quantitation, CSF and Amino Acids Quantitation, Plasma should be ordered at the same time to ensure collection of both specimens occurs within 30 minutes of each other.

Collection Alert:

Ideally, the blood specimen for Amino Acids Quantitation, Plasma should be collected just prior to the CSF collection, but is acceptable within 30 minutes of each other, for calculation of the CSF/plasma glycine ratio.

LABID (Connect Care):

LAB4009

Test Code:

CALGARY ZONE	BGLBL - IN BGL TEST NAME FIELD ENTER AMINO ACIDS CSF
CENTRAL ZONE	AACSF
EDMONTON ZONE	SFAAQ
NORTH ZONE	AACSF
SOUTH ZONE, LETHBRIDGE	MISC
SOUTH ZONE, MEDICINE HAT	AACSFS

Specimen Type:

Cerebral Spinal (a.k.a, Cerebro Spinal) Fluid (CSF)

Primary Container:

Sterile container

Minimum Collection Volume:

0.25 mL CSF

Specimen Collection Requirements:

Preference is to use the CSF collection tube with no visible blood contamination.

Test Resources:

Submit request using the applicable requisition or Connect Care online order entry:

Biochemical Genetics Laboratory Requisition

Please ensure all required information, including patient's clinical history/indication, is provided on the requisition.

Stability and Storage:

Frozen

Processing:

1. Centrifuge.
2. Transfer CSF supernatant to aliquot tube.
3. Indicate “CSF” on aliquot label.
4. Freeze aliquot tube immediately.

Transportation:

Transport frozen specimens on dry ice.
Samples must not thaw during transport.

Method:

Ion exchange liquid chromatography

Method and Interpretation of Results:

Amino acids are detected using an Amino Acid Analyzer with internal standard.

Various medications can lead to ninhydrin-positive peaks that interfere with the identification and quantitation of certain amino acids. It is recommend that all current medications being taken at the time of specimen collection be provided in the reason for referral.

Routine Turn Around Time:

7 days

Testing Schedule:

Daily

Reference Interval:

Reference intervals are age-dependent and will be provided on the report.

Testing Area:

Biochemical Genetics

Performing Site:

University of Alberta Hospital or Alberta Children’s Hospital

Additional Test Information:

For priority turn-around contact Biochemical Genetics Laboratory.

Contact Comments:

Biochemical Genetics North	Biochemical Genetics South
780-407-8820	403-955-7379

Last Updated On:

Tuesday, November 22, 2022

Date of Last Review:

Aug 1 2022 12:00AM