Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

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Test Name/Synonym:

Testosterone, Total

Clinical Indications:

This test is useful in the diagnosis of hypogonadism in males, androgen overproduction in females, and delayed or precocious puberty in children.

LABID (Connect Care):

LAB124

Specimen Type:

Blood

Primary Container:

Calgary Zone, South Zone: MINT LIHEP PST
Edmonton Zone: GOLD SST

Alternate Containers:

Calgary Zone, South Zone: GOLD SST
Edmonton Zone: MINT LIHEP PST

Minimum Collection Volume:

0.5 mL plasma or serum

Test Resources:

Biotin Interference

	General Laboratory Requisition – 21302
	Calgary Zone: Microcollection Minimum Whole Blood Volumes

Method and Interpretation of Results:

Testosterone exhibits diurnal variation in adult males.
Values are highest in the morning, with decreases up to 25% later in the day.
The amplitude of diurnal variation decreases with age, with patients aged greater than 70 showing an approximate 10% decrease. Diurnal variation of testosterone in females is not well defined in the literature, but similar variation may be expected in pre-menopausal women.
Reference: Brambilla, DJ, Matsumoto, AM, Araujo, AB, McKinlay, JB. The Effect of Diurnal Variation on Clinical Measurement of Serum Testosterone and Other Sex Hormone Levels in Men. J. Clin. Endocrinol. Metab. 2009. 94(3):907-913.

Testing Schedule:

Daily

Reference Interval:

Age	Female (nmol/L)	Male (nmol/L)	Unknown
0 up to 6 months	Less than 2.0	Less than 19.0	Interpretive comment includes male and female reference intervals
6 months up to 11 years	Less than 0.2	Less than 2.0
11 years up to 18 years	Less than 1.8
11 years up to 15 years		Less than 20.0
15 years up to 18 years		4.0 – 27.0
18 years up to unspecified	Less than 2.0	8.0 – 35.0

Testing Area:

Biochemistry

Performing Site:

Calgary:	Diagnostic and Scientific Centre
Edmonton:	Edmonton Base Lab

Last Updated On:

Thursday, December 11, 2025