Alberta Precision Laboratories

--- Query Results ---

Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

N-Acetylaspartic Acid (Synonym: Canavan Disease, Aminoacylase 2 Deficiency, Aspartoacylase Deficiency)

Clinical Indications:

This test is for the investigation of Canavan disease.

Test Includes:

Metabolites reported: N-Acetylaspartic Acid

Ordering Alert:

This test is considered specialty testing; this test will be cancelled if sufficient clinical justification is not provided.

This test should not be ordered in conjunction with Organic Acids, Semi-Quantitative, as this metabolite is also reported as part of that test, and this test will be reflexively performed on any urine sample with elevated levels detected.

LABID (Connect Care):

LAB4028A

Test Code:

CALGARY ZONE	BGLNON; IN BGL TEST NAME FIELD ENTER N-ACETYLASPART
OTHER ZONES	NOT SPECIFIED

Specimen Type:

Urine

Primary Container:

Sterile container

Alternate Containers:

Non sterile container

Minimum Collection Volume:

1.0 mL

Specimen Collection Requirements:

First morning void is preferred.
Collect a 5.0 to 10.0 mL random urine specimen.

Test Resources:

Submit request using the applicable requisition or Connect Care online order entry:

Biochemical Genetics Laboratory Requisition

Please ensure all required information, including patient's clinical history/indication, is provided on the requisition.

Stability and Storage:

Frozen

Processing:

This test requires creatinine determination for result reporting. For sites using EPIC, a separate aliquot should be removed at the time of initial specimen receipt and sent to the EPIC-designated lab for creatinine testing.

One aliquot with primary container, or two separate aliquots, can be submitted. Write “urine” on all secondary aliquot tubes. Freeze container and/or tubes.

Transportation:

Transport frozen specimens on dry ice.
Samples must not thaw during transport.

Method:

Liquid chromatography tandem mass spectrometry (LC-MS/MS)

Method and Interpretation of Results:

N-acetylaspartic acid is detected using liquid chromatography, electrospray-ionization tandem mass spectrometry (LC-MS/MS) with a deuterium-labelled internal standard.

N-acetylaspartic acid is significantly elevated in neonatal/infantile (i.e., severe) Canavan disease, but may be only marginally elevated in mild/juvenile Canavan disease.

Routine Turn Around Time:

30 days

Testing Schedule:

Monthly

Reference Interval:

Metabolite	Normal Range	Units
N-Acetylaspartic Acid	2.7 - 38.3	mmol/mol creatinine

Testing Area:

Biochemical Genetics

Performing Site:

Alberta Children’s Hospital

Additional Test Information:

For priority turn-around contact Biochemical Genetics South Laboratory.

Contact Comments:

Biochemical Genetics 403-955-7379

Last Updated On:

Monday, September 19, 2022

Date of Last Review:

Aug 1 2022 12:00AM