Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Test Name/Synonym:

Hereditary Spherocytosis by Flow Cytometry (Synonym: EMA, Eosin 5-Maleimide Binding Assay, HS, Osmotic Fragility, RBC Membranopathy, RBC Band 3)

Ordering Alert:

CBC and Reticulocyte Count must also be ordered.

Please refer to specimen stability and testing schedule.

Indicate RBC transfusion history.
Do NOT test if RBC transfusion within 3 months.

Collection Alert:

Edmonton: Collect Monday to Thursday ONLY. Do not collect the day before a weekend or a statutory holiday.

Shipping and Handling Alert:

Edmonton: Specimens must be received in the laboratory within 24 hours of collection and by 12:00 noon on the day before a weekend or a statutory holiday.

LABID (Connect Care):

LAB1134

Specimen Type:

Blood

Primary Container:

Calgary: PALE YELLOW (ACD-A) for Hereditary Spherocytosis and Lavender EDTA for CBC/Retic

Edmonton: LAVENDER EDTA

Alternate Containers:

Calgary: LAVENDER EDTA or LT BLUE CITRATE for Hereditary Spherocytosis and Lavender EDTA for CBC/Retic

Minimum Collection Volume:

0.5 mL

Specimen Collection Requirements:

CBC and Reticulocyte Count must also be collected.

Calgary Zone:
Adult: One 8.5 mL PALE YELLOW (ACD-A)
Pediatric: One 1.8 mL LT BLUE CITRATE

Test Resources:

Flow Cytometry Requisition (22229)

Stability and Storage:

Calgary:
Refrigerated - 7 days

Edmonton:
Refrigerated - 48 hours
Room temperature - 24 hours

Processing:

All specimens must be accompanied by a stained peripheral blood smear (PBS) slide.

DO NOT centrifuge or freeze specimen.

Transportation:

Within Calgary Zone:Transport at room temperature.
Within Edmonton Zone: Transport at refrigerated temperature.

For testing sent to Calgary from outside Alberta:

Transport at refrigerated temperature by FedEx overnight shipping. Specimen must be received within 24 hours of collection. Fax the tracking number and requisition to 403-270-4135.
Ship directly to:
Flow Cytometry Laboratory
Arthur J.E Child Comprehensive Cancer Centre
3395 Hospital Drive N.W. Calgary, Alberta T2N 5G2
Tel: 587-231-3904 Fax: 587-231-3910
APLFlowCytometry@albertaprecisionlabs.ca

For testing sent to Edmonton from outside Alberta:

Transport at refrigerated temperature.
Ship directly to:
Specimen Control, Laboratory Medicine and Pathology
4th Floor Walter Mackenzie Centre (4B2.10)
University of Alberta Hospital
8440 112 Street NW, Edmonton, AB, Canada T6G 2B7

Testing Schedule:

Calgary: Monday
Edmonton: Monday - Friday

Reference Interval:

See laboratory report.

Testing Area:

Flow Cytometry

Performing Site:

Calgary: Arthur Child Cancer Centre
Edmonton: University of Alberta Hospital

Contact Comments:

1-877-868-6848

Last Updated On:

Monday, March 17, 2025