Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

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Test Name/Synonym:

Testosterone, Free (Synonym: FTEST, TTF)

Test Includes:

Free Testosterone is a calculation involving measurement of Total Testosterone, Albumin, and Sex Hormone Binding Globulin.

LABID (Connect Care):

LAB1756

Specimen Type:

Blood

Primary Container:

Calgary: MINT LIHEP PST
Edmonton: GOLD SST

Alternate Containers:

Calgary: GOLD SST
Edmonton: MINT LIHEP PST

Minimum Collection Volume:

Calgary: 0.5 mL serum or plasma
Edmonton: 1 mL serum or plasma

Test Resources:

General Laboratory Requisition – 21302 – write test name under “Additional Tests”

Calgary Zone: Microcollection Minimum Whole Blood Volumes

Processing:

Tested at UAH (SHBG and FTEST): Centrifuge, aliquot, and freeze

Transportation:

Tested at UAH – transport frozen

Testing Schedule:

Tested at DSC or EBL:Daily
Tested at UAH: Monday to Friday

Reference Interval:

Age	Gender	pmol/L
Less than 11 years	All	No reference interval
11 years up to 18 years	All	No Reference Interval Clinical correlation with puberty status suggested
18 years up to unspecified	Female	2 – 30
	Male	175 – 700
	Unknown/X	Interpretive comment includes male and female reference intervals.

Reference intervals were established on specimens collected in the morning (07:00 - 10:00), therefore it is suggested to collect specimens within this timeframe.

Testing Area:

Biochemistry

Performing Site:

Calgary:	Diagnostic and Scientific Centre
Edmonton:	Edmonton Base Lab
Edmonton:	University of Alberta Hospital

Additional Test Information:

This test is performed by DSC and EBL Biochemistry and Edmonton UAH Special Chemistry.

The concentration of Free Testosterone is derived from a mathematical expression based on constants for the binding of Testosterone to Sex Hormone Binding Globulin and Albumin.

Testosterone exhibits diurnal variation in adult males.
The amplitude of diurnal variation decreases with age, with patients aged greater than 70 years showing an approximate 10% decrease.
Diurnal variation of testosterone in females is not well defined in the literature, but similar variation may be expected in pre-menopausal women.

Reference:
Brambilla, DJ, Matsumoto, AM, Araujo, AB, McKinlay, JB. The Effect of Diurnal Variation on Clinical Measurement of Serum Testosterone and Other Sex Hormone Levels in Men. J. Clin. Endocrinol. Metab. 2009. 94(3):907-913

Last Updated On:

Thursday, December 11, 2025