Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

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Test Name/Synonym:

Pyruvate, Blood Panel (Synonym: Lactate/Pyruvate Ratio, Pyruvic Acid)

Pathogen:

Disorders of mitochondrial metabolism or unexplained refractory epilepsy.

Clinical Indications:

Measurement of blood pyruvate, within the context of persistently elevated lactate, is used to distinguish between pyruvate dehydrogenase deficiency and other causes of congenital lactic acidosis. This test has no clinical utility outside of the context of elevated lactate.

Test Includes:

Pyruvate and Lactate/Pyruvate Ratio are reported.

Ordering Alert:

This test must be pre-booked with the Alberta Children’s Hospital (ACH) Biochemical Genetics Laboratory 403-955-7379 or with the University of Alberta Hospital Special Chemistry Laboratory 780-407-8491 at least 24 hours in advance.

This test is considered specialty testing; this test will be cancelled if sufficient clinical justification is not provided. Test will NOT be performed if patient has NOT had previous evidence of elevated blood or CSF lactate level.

This test must be ordered in conjunction with Lactate, Blood. Both tests (Pyruvate and Lactate) will be performed on the same specimen. Arterial or venous blood must be specified for correct reference range to apply to results.

Collection Alert:

The patient must be fasting for at least 4 hours (if possible, discontinue IV dextrose) before collection. The patient must avoid vigorous exercise for at least 30 minutes before collection.

Alberta Children’s Hospital collections:

Collection can ONLY be performed onsite at Alberta Children’s Hospital Outpatient lab.
Biochemical Genetics Laboratory staff MUST be called before collection.

University of Alberta Hospital collections

Special tubes are required for collection. Refer to Specimen Collection Requirements.
Special Chemistry Laboratory staff MUST be called before collection.
Outpatient collection is by appointment only at the Edmonton Kaye Clinic; Special Chemistry must be informed and present for collection.

LABID (Connect Care):

LAB7744

Test Code:

CALGARY
BGLBL: IN BGL TEST NAME FIELD ENTER “PYRUVATE”
AND
LACT (IF LACTATE HAS NOT ALREADY BEEN ORDERED FOR THIS COLLECTION)

Specimen Type:

Blood

Specimen Source:

Alberta Children’s Hospital collections: Venous blood
University of Alberta Hospital collections: Arterial or venous blood

Primary Container:

CALL LAB

Minimum Collection Volume:

1.0 mL whole blood, 2.0 mL preferred.

Specimen Collection Requirements:

Alberta Children’s Hospital collections:

Do not collect until Biochemical Genetics staff is present.

University of Alberta Hospital collections:

Arterial blood (collected by a doctor or nurse) is the preferred specimen.
Do not collect until Special Chemistry Laboratory staff is present with acid containing collection tubes.

Test Resources:

Submit request using requisition or Connect Care online order entry:

General Laboratory Requisition – 21302– write test name under “Additional Tests”.

Indicate length of fast on the requisition (with collection time).

Processing:

Alberta Children’s Hospital collections:

The Biochemical Genetics Laboratory staff member present for collection will process the specimen.

University of Alberta Hospital collections:

The Special Chemistry Laboratory staff member present for collection will process the specimen.
If collection is offsite, processing instructions will be sent with collection tubes.

Transportation:

Transport frozen

Method:

Spectrophotometric

Routine Turn Around Time:

Within 3 days

Testing Schedule:

Prearranged

Reference Interval:

Test	Age		Reference Interval	Units
Pyruvate	Less than 3 years	Arterial blood	60-150	µmol/L
	Greater than 3 years	Arterial blood	50-80	µmol/L
	Greater than 3 years	Venous blood	30-100	µmol/L
Lactate/Pyruvate Ratio	All		Less than 25

Testing Area:

Biochemistry

Performing Site:

Edmonton Zone: University of Alberta Hospital

Additional Test Information:

This test is performed by Edmonton Special Chemistry.

Contact Comments:

Alberta Children’s Hospital Biochemical Genetics 403-955-7379
University of Alberta Special Chemistry 780-407-8491

Last Updated On:

Thursday, October 6, 2022