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| Organization: | Alberta Precision Laboratories | ||||||
| Message Alert: | PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing. | ||||||
| Test Name/Synonym: | Anti-Neutrophil Cytoplasmic Antibody (ANCA) (Synonym: ANTI MPO, ANTI-PR3 ANTIBODY, MPO ANTIBODY, PR3 ANTIBODY, PR3 ANTIBODY, PROTEINASE 3 ANTIBODY, ANCAS, cANCA, pANCA, antineutrophil cytoplasmic antibody, Wegener's, myeloperoxidase antibody, granulomatosis with polyangitis, microscopic polyangitis, eosinophilic granulomatosis with polyangitis) | ||||||
| Clinical Indications: | Evaluating patients suspected of having anti-neutrophil cytoplasmic antibody (ANCA) associated vasculitis. | ||||||
| Test Includes: | This test includes p-ANCA, c-ANCA, Anti-MPO, Anti-PR3 and atypical ANCA. | ||||||
| LABID (Connect Care): | LAB458 | ||||||
| Specimen Type: | Blood | ||||||
| Specimen Source: | Venous | ||||||
| Primary Container: | GOLD SST | ||||||
| Alternate Containers: | RED RAPID SERUM TUBE | ||||||
| Minimum Collection Volume: | ANCA: 0.5 mL serum MPO and PR3: 0.5 mL serum | ||||||
| Specimen Collection Requirements: | Calgary Zone: Two GOLD SST tubes Edmonton: One GOLD SST tube | ||||||
| Test Resources: |
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| Stability and Storage: | Calgary: Primary tube stable for 5 days in the fridge. Edmonton: Serum aliquot stable for 7 days in the fridge. Extended stability if frozen. Avoid multiple freeze thaw cycles. | ||||||
| Processing: | Edmonton Zone: Aliquot serum into polypropylene or polystyrene tube and freeze. | ||||||
| Transportation: | For testing sent to Calgary Zone: Transport at refrigerated or room temperature. For testing sent to Edmonton Zone: Transport frozen. | ||||||
| Method: | Indirect immunofluorescence assay IFA (ANCA slide) Multiplex bead immunoassay (anti-MPO, anti-PR3) | ||||||
| Method and Interpretation of Results: | Positive results for antineutrophil cytoplasmic antibodies (ANCA) demonstrate 2 main patterns, cytoplasmic (C-ANCA) and perinuclear (P-ANCA). Negative ANCA results do not rule out a diagnosis of ANCA-associated vasculitis. Due to lack of diagnostic specificity, all positive ANCA results are confirmed using an antigen specific immunoassay using proteinase 3 ANCA for cytoplasmic ANCA and myeloperoxidase ANCA for perinuclear ANCA. Atypical ANCA patterns are infrequent comprising a mix of cytoplasmic and perinuclear fluorescence often with multiple antigen specificities and unclear clinical significance. Some ANA Patterns (homogenous, speckled, dense fined speckled DFS, and centromere) may be associated with false pANCA pattern. | ||||||
| Routine Turn Around Time: | 1 - 3 days | ||||||
| Testing Schedule: | Monday to Friday | ||||||
| Reference Interval: |
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| Testing Area: | Biochemistry | ||||||
| Performing Site: | Calgary: Diagnostic and Scientific Centre Edmonton: University of Alberta Hospital | ||||||
| Additional Test Information: | Testing is performed in Calgary Immunochemistry and Edmonton Special Chemistry. For urgent requests, contact the on-call clinical biochemist. | ||||||
| Last Updated On: | Tuesday, October 22, 2024 | ||||||
