--- Query Results --- | |||||||||||||||||||||||||||||||||||||||
| Organization: | Alberta Precision Laboratories | ||||||||||||||||||||||||||||||||||||||
| Test Name/Synonym: | Crithidia dsDNA Antibody (Synonyms: Double-stranded dna antibodies, Anti-dsdna antibodies, Dsdna antibodies, Anti dna antibodies) | ||||||||||||||||||||||||||||||||||||||
| Clinical Indications: | Antibodies to dsDNA help confirm the diagnosis of Systemic Lupus Erythematosus in patients with clinical features. | ||||||||||||||||||||||||||||||||||||||
| Ordering Alert: | If a patient’s Anti-dsDNA Bioplex result does not fit the clinical picture, page the on-call clinical biochemist to request add-on for dsDNA antibody by Crithidia IFA. | ||||||||||||||||||||||||||||||||||||||
| Shipping and Handling Alert: | Avoid multiple freeze thaw cycles. | ||||||||||||||||||||||||||||||||||||||
| LABID (Connect Care): | LABID6162 | ||||||||||||||||||||||||||||||||||||||
| Specimen Type: | Blood | ||||||||||||||||||||||||||||||||||||||
| Specimen Source: | Venous | ||||||||||||||||||||||||||||||||||||||
| Primary Container: | GOLD SST | ||||||||||||||||||||||||||||||||||||||
| Alternate Containers: | RAPID SERUM TUBE RED | ||||||||||||||||||||||||||||||||||||||
| Minimum Collection Volume: | 0.6 mL serum | ||||||||||||||||||||||||||||||||||||||
| Specimen Collection Requirements: | One 5 mL RAPID SERUM TUBE is acceptable within one hour of closing. | ||||||||||||||||||||||||||||||||||||||
| Test Resources: |
Stability and Storage: | Serum aliquot stable for 7 days in the fridge. Extended stability if frozen. Avoid multiple freeze thaw cycles. | Processing: | Centrifuge and aliquot serum into polypropylene or polystyrene tube and freeze. | Method: | Crithidia indirect immunofluorescence assay (IFA slide) | Available for indeterminant multiplex bead immunoassay (Bioplex) anti-dsDNA results that do not fit the clinical picture. Method and Interpretation of Results: | Negative: Testing for dsDNA antibody by Crithidia IFA was negative. Unable to confirm positive result obtained by multiplex immunoassay. | Positive: Testing for dsDNA antibody by Crithidia IFA was positive. Confirmed positive result obtained by multiplex immunoassay. Weak Positive: Testing for dsDNA antibody by Crithidia IFA was weakly positive. Indeterminant Routine Turn Around Time: | 7 days | | Testing Schedule: | Once a week | | Reference Interval: | Negative | Testing Area: | Biochemistry | | Performing Site: | Diagnostic and Scientific Centre | University of Alberta Hospital Additional Test Information: | Testing performed in Calgary Immunochemistry and Edmonton Special Chemistry | | Last Updated On: | Monday, March 24, 2025 | | ||||||||||||
