Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

Breast/Ovarian/Prostate Cancer Panel (Synonyms: ATM, BARD1, BRCA1, BRCA2, BRIP1, CDH1, CHEK2, EPCAM, MLH1, MSH2, MSH6, PALB2, PMS2, PTEN, RAD51C, RAD51D, STK11, TP53, Inherited Cancer and Endocrine Gene Panel)

Test Includes:

Genes: ATM, BARD1, BRCA1, BRCA2, BRIP1, CDH1, CHEK2, EPCAM, MLH1, MSH2, MSH6, PALB2, PMS2, PTEN, RAD51C, RAD51D, STK11, TP53

Ordering Alert:

Ordering is Restricted:

Confirmation of Diagnosis¹

Clinical Genetics
Oncology clinicians who have had mainstreaming orientation from Hereditary Cancer Clinic Hub

Presymptomatic Testing² Clinical Genetics

¹patient has signs or symptoms of the disease/disorder
²patient does not presently have symptoms, positive family history of the condition

Collection Alert:

Unopened dedicated tubes (do not share) are required for Molecular Genetics testing.

LABID (Connect Care):

LAB12566

Specimen Type:

Blood, Swab, Tissue, DNA, Other

Specimen Source:

Indicate a specimen source.

Primary Container:

Lavender top EDTA

Alternate Containers:

Microcollection EDTA

Minimum Collection Volume:

See Specimen Collection Requirements

Specimen Collection Requirements:

For Blood Specimen (peripheral)
EDTA tubes should be completely full, 2/3 full at minimum, testing may be affected by partial draw

For Patients over 20 kg: 2 EDTA tubes each containing 3.0 mL of blood
For Patients up to 20 kg: 2 EDTA tubes each containing 2.0 mL of blood

For Other Tissue Specimens

For Fresh or Frozen Tissue specimens: Submit a minimum of 20 mg in a sterile container with NO fixative.
For Cultured Cells (for Molecular Genetics South only) - Confluent (overgrown) T25 flask (or equivalent) placed in transport media.

For Extracted DNA

Submit 10ug @ >100ng/uL.

For Buccal Swab Specimens

Collect 4 buccal brushes.Unopened dedicated tubes (do not share) are required for Molecular Genetics testing.

Test Resources:

Pre-test Counselling Information-Genetic and GenomicTesting

Breast/Ovarian/Prostate Cancer NGS Panel Information Sheet

Submit requests using requisition or Connect Care online order entry:

Molecular Genetics Cancer and Endocrine Next Generation Sequencing Requisition

Please ensure all required information, including patient's clinical history/indication, is provided on the requisition.

Stability and Storage:

Room temperature: For all specimen types (except frozen tissue) if being shipped on same day of collection (preferred).
Refrigerated: If shipping is delayed.
Frozen: Frozen tissue specimens at -20^oC to -80^oC.

Processing:

Do not centrifuge or aliquot specimens.

Transportation:

Transport all specimen types (except frozen tissue) at room temperature.
Specimens must not freeze in transit.

Transport frozen tissue specimens with adequate amounts of dry ice to keep specimen frozen.
Specimens must not thaw in transport.

Specimens, should be received by the Molecular Genetics Laboratory:

Within 5 days of date of collection for Blood and Extracted DNA specimens.
Within 24 hours of date of collection for Tissue specimen types.
And must include the original paper requisition and accompanying documentation.

Submitting to Molecular Genetics North Transport specimens (except extracted DNA) to Molecular Genetics Laboratory via UAH Specimen Control WMC 4B2.10.

For Extracted DNA samples only, transport specimens to Molecular Genetics Laboratory via UAH Shipping Receiving, UNIVERSITY OF ALBERTA HOSPITAL, 8440 112 STREET, Room: 8-26 MSB, EDMONTON, T6G 2B7

Blood,Tissue, Buccal Swab, and Extracted DNA specimens

Are received Mon – Fri: 07:30 to 15:00.
If received after 15:00, will be processed on the next working day.

If Tissue specimens are sent from outside of Edmonton for testing in Molecular Genetics North, please call the laboratory at 780-407-1434 or fax shipping information to 780-407-1761 to inform the lab of expected arrival times and mode of transport.

Submitting to Molecular Genetics South
Transport specimens to Molecular Genetics Laboratory at Alberta Children’s Hospital Room B0-020.

Method:

Next Generation Sequencing.

Method and Interpretation of Results:

Genomic DNA is analyzed using next generation sequencing technology (NGS). NGS detects nucleotide substitutions, small insertions and deletions, and copy number variants. If a variant is clinically relevant or of uncertain significance and does not meet our validated analytic requirements it is confirmed by an appropriate molecular method. Sanger sequencing is used to analyze regions which do not meet requirements for depth of sequence coverage.

EPCAM (exon copy number only).

PMS2 by MLPA: Detects deletions and duplications. Copy number analysis by MLPA is completed on exons 11-15 only if clinically indicated. i.e. IHC results.

Routine Turn Around Time:

Routine: up to 16 weeks
Stat: 4 weeks

Reference Interval:

Interpretation provided on report.

Testing Area:

Molecular Genetics

Performing Site:

Univeristy of Alberta Hospital or Alberta Children's Hospital

Additional Test Information:

To obtain buccal swab collection kits, contact:

Molecular Genetics North	Molecular Genetics South
780-407-1434	403-955-3097

Contact Comments:

For additional information refer to Genetics and Genomics webpage or contact:

Molecular Genetics North	Molecular Genetics South
780-407-1434 (phone) 780-407-1761 (fax)	403-955-3097 (phone) 403-955-7624 (fax)

Last Updated On:

Monday, October 21, 2024