Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

CBFB::MYH11 Inversion (16), Quantitative [Synonyms: Inv(16), t(16;16), Inv (16)(p13.1q22), t(16;16)(p13.1q22)]

Clinical Indications:

Useful for patients with acute myeloid leukemia at the time of diagnosis as well as for minimal residual disease monitoring during the clinical and therapeutic course of these patients.

Test Includes:

Gene: CBFB::MYH11

Ordering Alert:

Test is restricted to hematologists and hematopathologists

LABID (Connect Care):

LAB9639

Specimen Type:

Bone marrow, Peripheral blood

Specimen Source:

Indicate specimen source.

Primary Container:

See Specimen Collection Requirements.

Specimen Collection Requirements:

Molecular Pathology North

DSC External Send-Out

Bone Marrow

Collect one EDTA (lavender top) tube containing a minimum of 2.0 mL of bone marrow (preferred volume is 4 mL) and
Collect one PAXgene^® Bone Marrow RNA tube containing a maximum of 2.0 mL of bone marrow. This tube is for the Molecular Pathology North lab.
Ensure to follow the specific collection requirements: Place 2.0 to 4.0 mL of bone marrow into the EDTA tube and not more than 2.0 mL of bone marrow into the PAXgene^® tube. Order of draw is not important.

Bone Marrow

Collect one EDTA (lavender top) tube containing a minimum of 2.0 mL bone marrow (preferred volume is 4 mL).
Only collect on Monday to Wednesday, preferably Monday and Tuesday.
Read transportation requirements before collection.

Peripheral Blood

Collect one EDTA tubes containing a minimum of 4.0 mL of blood and
Collect one PAXgene^® Blood RNA tube containing a minimum of 2.5 mL of blood.
An additional EDTA tube will be required for CBC testing if no CBC has been done in the last month (reflex action in Epic Beaker).
Ensure to follow the specific specimen collection requirements: Using a butterfly needle and holding the tubes below the arm, first collect the EDTA tube(s), then the PAXgene^® Blood RNA tube.
Collection must be in the correct order of draw to ensure accurate white count determination.
Due to limited stability, samples for this test should not be collected the day before a holiday or 3-day weekend.

Peripheral Blood

Collect two EDTA (lavender top) tubes containing a minimum of 4.0 mL of peripheral (preferred minimum total volume is 10 mL).
Collect Mondays to Wednesday, preferably Monday and Tuesday.
Read transportation requirements before collection

Test Resources:

Edmonton: Submit requests using Molecular Pathology Requisition or Connect Care online order entry.

Please ensure all required information, including patient's clinical history/indication, is provided on the requisition.

Calgary: Connect Care online order entry

Stability and Storage:

University Health Network: Extracted RNA samples will be sent for Edmonton cases.

Mayo Clinical Laboratories: stability (from collection to initiation of testing): 5 days refrigerated, 3 days ambient.

Processing:

Molecular Pathology North	DSC External Send-Out
Immediately following collection, place all tubes ON ICE or COLD PACK. Do not centrifuge, aliquot or freeze. Due to limited stability, samples for this test should not be collected the day before a holiday or 3-day weekend. Send EDTA tube for a CBC to be processed as per local handling practice (only if no CBC has been done in the last month). This is a reflex action in Epic Beaker.	Immediately following collection, place all tubes ON ICE or refrigerate. Do not centrifuge, aliquot or freeze.

Transportation:

Molecular Pathology North	DSC External Send-out
Transport specimens as soon as possible, on ice or cold pack. Molecular Pathology must receive the specimens within 24 hours of collection. If transport is anticipated to be greater than 24 hours after collection, contact Molecular Pathology. Extracted RNA samples (shipped frozen on dry ice) will be sent to the University Health Network (UHN).	Transport specimens as soon as possible on ice or cold pack Specimen must be received in DSC External Send Out Department by 13:00 the day following collection. Refrigerated specimens must arrive within 5 days of collection, and ambient specimens must arrive within 3 days of collection at Mayo Clinic Laboratories.

Method:

Quantitative real-time reverse transcription PCR

Method and Interpretation of Results:

University Health Network (UHN): unkown

Mayo Clinic Laboratories: Total RNA is extracted from blood or bone marrow and reverse transcribed to generate complementary DNA. Quantitative real-time reverse transcription polymerase chain reaction is performed using the LightCycler instrument platform, and the data analyzed using the dedicated software for relative quantification with calibrator normalization. Results are provided as a normalized relative value of CBFB::MYH11::ABL1 messanger RNA transcripts with a reproducible analytical sensitivity of 0.01% (Unpublished Mayo method).

The normalized ratio is a relative quantification calculation as follows:

Normalized ratio =
CBFB::MYH11 (sample)/ABL1 (sample)/
CBFB::MYH11 (run calibrator)/ABL1 (run calibrator)

Routine Turn Around Time:

University Health Network: Unknown
Mayo Clinic Laboratories: 4-8 days

Testing Schedule:

University Health Network: Unknown
Mayo Clinic Laboratories: Performed Monday through Saturday

Testing Area:

Edmonton: University Health Network (UHN)
Calgary: Mayo Clinic Laboratories

Performing Site:

Edmonton: Toronto
Calgary: Rochester

Additional Test Information:

Edmonton:
PAXgene^® collection tubes are available upon request.
Submit an order as per online Client Order Form Submission process.
Details in following document: RR01-005 Ordering Supplies from Reagent Room

Contact Comments:

Molecular Pathology North: 780-407-6648
Moelcular Pathology South: 587-231-3199

Last Updated On:

Friday, December 20, 2024

Registered Trademark Comments:

PAXgene^® is a trademark of PreAnalytiX GmbH.