Alberta Precision Laboratories

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Organization:

Sub-Page

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

Blood Gas, Venous – ABL 90 Flex Plus (Synonym: VBG, Venous Blood Gas, BG)

Test Includes:

pO2, O2 Saturation, and Oxyhemoglobin will not be reported on specimens collected in vacutainers.

All zones: pH, pCO2, pO2, O2 Saturation, HCO3, Base Excess, Total Hemoglobin, Oxyhemoglobin, Carboxyhemoglobin, Methemoglobin, Hematocrit, Lactate, Glucose, Sodium, Potassium, Chloride, Anion Gap, Ionized calcium, Ionized calcium normalized, and Barometric Pressure.

Ordering Alert:

These tests are run as a panel only. This test panel is NOT for routine electrolyte, metabolite, or hemoglobin testing.

Collection Alert:

For community or outpatient requests, refer to Blood Gas, Venous – Community and Outpatients

Do not collect at a Patient Service Centre or by Mobile Collection Services.

If a tourniquet is used during collection, refer to specimen collection requirements below for collection guidelines.

LABID (Connect Care):

LAB79

Specimen Type:

Blood

Specimen Source:

Venous

Primary Container:

Edmonton Zone:HEPARINIZED SYRINGE
South Zone: DARK GREEN – LIHEP NO GEL

Alternate Containers:

South Zone: HEPARINIZED SYRINGE

Minimum Collection Volume:

Vacutainers must be filled to capacity
HEPARINIZED SYRINGE: 0.7 mL whole blood.
South Zone: Pediatric NICU specimens 0.5 mL whole blood

Specimen Collection Requirements:

A tourniquet is not required for collection but can be used during collection if the following guidelines are followed:

Draw sample immediately after application of tourniquet; tourniquet application must not exceed one minute before accessing vein.
If tourniquet is in place longer than one minute before accessing vein, release it and reapply after two minutes before performing venipuncture.
Do not clench fist during and prior to collection.

Frozen, clotted, or specimens containing air bubbles will be rejected.

Stability and Storage:

Edmonton Zone:
Room temperature: 30 minutes
South Zone:
If Lactate reported:
Room temperature: 15 minutes
If Lactate not reported:
Room temperature: 60 minutes

Processing:

Avoid / remove air bubbles from heparinized syringes.
Mix well by gentle inversion.

Transportation:

Transport to performing department immediately.
Transport at room temperature.

Testing Schedule:

Daily

Reference Interval:

Parameter	Units	Gender	Reference Interval
pH	N/A	All	7.30 – 7.40
pCO2	mmHg		35 – 50
pO2	mmHg		30.0 – 50.0
HCO3	mmol/L		20 – 27
BE	mmol/L		-4 - 1
tHb	g/L		Refer to CBC and Differential Provincial Reference Intervals and Critical Values - Epic (albertahealthservices.ca)
O2 Sat	%		50 – 80
COHb	%		0.0 – 3.0
MetHb	%		0.0 – 1.5
OxyHb	%		50 - 80
Lactate	mmol/L		Refer to individual analyte test page
Glucose	mmol/L		Refer to individual analyte test page
Ionized Calcium	mmol/L		Refer to individual analyte test page
Ionized Calcium normalized	mmol/L		Refer to individual analyte test page
Sodium	mmol/L		Refer to individual analyte test page
Potassium	mmol/L		Refer to individual analyte test page
Chloride	mmol/L		Refer to individual analyte test page
Anion gap	mmol/L		Refer to Electrolyte Panel test page
Hct	L/L		Refer to CBC and Differential Provincial Reference Intervals and Critical Values - Epic (albertahealthservices.ca)

Critical Value:

Parameter	Units	Gender	Critical Value
pH	N/A	All	Less than 7.15 or greater than 7.55
pCO2	mmHg		Less than 15, or if greater than 55 when pH is less than 7.20
HCO3	mmol/L		Less than 10 or greater than 40
tHb	g/L		Refer to CBC and Differential Provincial Reference Intervals and Critical Values - Epic (albertahealthservices.ca)
COHb	%		Greater than 15
MetHb	%		Greater than 10
Lactate	mmol/L		Refer to individual analyte test page
Glucose	mmol/L		Refer to individual analyte test page
Ionized Calcium	mmol/L		Refer to individual analyte test page
Ionized Calcium normalized	mmol/L		Refer to individual analyte test page
Sodium	mmol/L		Refer to individual analyte test page
Potassium	mmol/L		Refer to individual analyte test page

Testing Area:

Biochemistry

Performing Site:

Edmonton Zone:	Fort Saskatchewan Community Hospital
South Zone:	Chinook Regional Hospital Medicine Hat Regional Hospital

Last Updated On:

Thursday, November 21, 2024