Alberta Precision Laboratories

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Test Name/Synonym:

Bacterial Culture, aerobic and anaerobic, and Fungal Culture - Bone Marrow - Calgary Zone (CLS)

Test Code:

BACTERIAL CULTURE: M ANO2
FUNGAL CULTURE: M FUNGAL

Primary Container:

Pediatric Aerobic (Yellow) and anaerobic (Orange) bottle and Sodium Polyanethol (SPS) tube

Specimen Collection Requirements:

Bone Marrow Culture includes aerobic, anaerobic and fungal cultures. Submit all 3 collection containers.

Aerobic Culture: Submit a minimum of 0.5 mL bone marrow in a yellow top (pediatric) blood culture bottle.
Anaerobic Culture: Submit a minimum of 0.5 mL bone marrow in a orange top blood culture bottle.
Fungal Culture: Submit 0.5 mL to 1 mL bone marrow in a Sodium Polyanethol (SPS) tube.


Aerobic Culture Bottle	Anerobic Culture Bottle	Fungal SPS Tube

Collection:

Check bottom of bottle - if it is yellow, DO NOT USE (the bottle may be contaminated)
Check expiry date(y/m/d) found on the blood culture bottle label and if expired, DO NOT USE.
Disinfect bottle tops with 70% isopropanol and allow to dry for 1 minute. DO NOT USE IODINE.
Health care Provider disinfects bone marrow aspiration site as per AHS Guidelines.
After collection, gently mix bottles by inversion to prevent clotting.
Ensure both bottles are labeled with required patient identifiers.

DO NOT COVER the 2D or other bottle barcodes by over labeling with patient identifier or PathNet labels.

Processing:

Store specimen at room temperature.

DO NOT refrigerate.

Testing Schedule:

Test performed on a daily basis.

Testing Area:

Microbiology

Performing Site:

Diagnostic and Scientific Centre

Additional Test Information:

Turnaround time for negative culture is 5 days for bacterial culture and up to 6 weeks for a negative fungal culture.