Alberta Precision Laboratories

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Test Name/Synonym:

Helicobacter pylori Culture (Gastric or Duodenal Biopsy) - Calgary Zone

Test Code:

M HPYLC

Primary Container:

Portagerm transport media

Specimen Collection Requirements:

Helicobacter pylori culture for susceptibility testing is performed ONLY if a history of treatment failure is indicated.

The following groups of medication should not be used for the period indicated before collecting the specimen for the Helicobacter pylori culture as these agents can affect the laboratory's ability to recover H.pylori:

Proton pump inhibitors (H+, K+ - ATPase Inhibitor):	14 days
Antibiotics:	28 days
Bismuth preparations, (e.g., Peptol Bismol):	14 days

Acceptable specimen: biopsy tissue.

The Day Before Biopsy Collection:

Obtain an H. Pylori Collection Kit (contains Portagerm transport media, requisitions, collection checklist and biohazard bags) from the hospital laboratory Accession Department. Keep the kit in the fridge overnight.

Aerobic

Portagerm transport media

Alternatively, Tryptic Soy Broth (TSB) media can be used and will be distributed in the event the Portagerm transport media is unavailable. Process for collection is the same as for Portagerm transport media.

Day of the Biopsy Collection:
1. Remove the Collection Kit from fridge and warm to room temperature prior to collecting samples.
2. Using Gastric or Duodenal Biopsy Collection Checklist for Helicobacter Culture, Guidelines for Physicians, MI6106, collect two double-bite biopsies:
  - One from the antrum near pylorus
  - One from the gastric body (greater curvature)
3. Place each biopsy into its own transport media container.
4. Label all specimens with patient name, hospital or Alberta health care number AND body site of biopsy (antrum or gastric body).
5. Place each biopsy into a separate biohazard bag.
6. Requisitions are provided in the kit for each specimen site. Label each requisition with:
  - Patient label with 2 unique identifiers
  - Ordering physician information
  - Date and time of collection

Processing:

Transport STAT to the hospital laboratory.

Send labelled specimens, requisitions and Gastric or Duodenal Biopsy Collection Checklist for Helicobacter Culture, Guidelines for Physicians, MI6106

Specimen MUST be received in Microbiology at the Diagnostic and Scientific Centre (DSC) within 2 hours of collection.

Testing Schedule:

Test performed on a daily basis.

Testing Area:

Microbiology

Performing Site:

Diagnostic and Scientific Centre

Additional Test Information:

Turnaround time for negative culture: 10 days.

See Helicobacter pylori Stool Antigen
See Urea Breath Test for Helicobacter pylori

Last Updated On:

Saturday, April 18, 2020