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| Organization: | Alberta Precision Laboratories | ||||||||
| Message Alert: | PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing. | ||||||||
| Test Name/Synonym: | Pneumocystis jirovecii (PJP NAT, PJP PCR, P. carinii, PCP) | ||||||||
| Pathogen: | Pneumocystis jirovecii pneumonia (PJP) | ||||||||
| Test Includes: | Nucleic acid amplification test (NAT) | ||||||||
| Shipping and Handling Alert: | Calgary Zone and South Zone testing is performed at APL-Calgary Microbiology (Diagnostic and Scientific Centre) Edmonton Zone, Central Zone and North Zone testing is performed at APL-Public Health Laboratory (Edmonton) | ||||||||
| LABID (Connect Care): | LAB906 (PNEUMOCYSTIS JIROVECII) | ||||||||
| Test Code: |
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| Specimen Type: | Bronchoalveolar Lavage Fluid (BAL) *preferred Bronchial Wash/Brushing Induced Sputum Endotracheal aspirate | ||||||||
| Primary Container: | Sterile Collection Container
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| Minimum Collection Volume: | 1-2 mL | ||||||||
| Test Resources: | Testing for Pneumocystis jirovecii is by real time Polymerase Chain Reaction (PCR). Bronchoalveolar Lavage Fluid is the preferred specimen type. For BAL specimens, molecular methods are 97-99% sensitive and 90-94% specific [1]. Bronchial Wash, induced sputum and ET aspirates are also accepted however the assay has not been validated for these specimen types and test performance has not been established [2]. For Edmonton Zone, Central Zone and North Zone, consult APL Public Health Microbiologist on Call prior to collection/submission of these non-validated specimen types (bronchial wash, induced sputum and ET aspirate). Note: Non-induced/expectorated sputum specimens are NOT acceptable | ||||||||
| Stability and Storage: | Calgary Zone/Edmonton Zone: Store specimen at room temperature. South Zone/Central Zone/North Zone: Refrigerate and transport on ice. | ||||||||
| Routine Turn Around Time: | Calgary : 24 hours Public Health (Edmonton): 24-48 hours | ||||||||
| Testing Schedule: | Calgary: Daily. Specimens that arrive after 12:00 (noon) will be run the following day. Public Health (Edmonton): 3 times per week | ||||||||
| Testing Area: | Microbiology | ||||||||
| Performing Site: | Calgary: Diagnostic and Scientific Centre Public Health: APL Public Health Edmonton (Provlab) | ||||||||
| Additional Test Information: | 1. Bateman et al. 2020. Diagnosing Pneumocystis jirovecii pneumonia: A review of current methods and novel approaches. Medical Mycology 58: 1010-1028 2. Senecal et al. 2021. Non-invasive diagnosis of Pneumocystis jirovecii pneumonia: a systematic review and meta-analysis. Clinical Microbiology and Infection https://doi.org/10.1016/j.cmi.2021.08.017 | ||||||||
| Last Updated On: | Friday, February 11, 2022 | ||||||||
