Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

Cell Free Nucleic Acid Analysis (Referred Test) (Synonym: T790M EGFR,
EGFR T790M Circulating Tumor DNA (ctDNA) Testing, Plasma CFDNA EGFR Mutation Testing)

Clinical Indications:

For investigation of lung cancer.

Collection Alert:

Unopened dedicated tubes are required for Molecular Pathology testing.
Restricted collection locations: See Specimen Collection Requirements.
Do not collect on Friday or prior to a public holiday.

Shipping and Handling Alert:

Time sensitive: Specimens must be received in the Molecular Pathology South within 72 hours of collection.

LABID (Connect Care):

LAB48510

Specimen Type:

Blood

Specimen Source:

Indicate specimen source.

Primary Container:

Black and brown top - STRECK Cell-Free DNA BCT^® (blood collection tube)

Minimum Collection Volume:

8.0 mL blood in each tube

Specimen Collection Requirements:

Molecular Pathology North	Molecular Pathology South
Specimens must be collected at Cross Cancer Institute outpatient laboratory. Collect two STRECK Cell-Free DNA blood collection tubes.	Specimens are collected at Foothills Medical Center (FMC) Special Services Building laboratory. Specimens can be collected at rapid response labs in Calgary. Contact Foothills Medical Center Accession to arrange for transport for the Streck tubes to the requesting site. Collect two STRECK Cell-Free DNA blood collection tubes.

When using a winged blood collection set (butterfly needle) a discard tube must be used if only a Streck Cell-Free DNA blood collection tube is drawn or is the first tube drawn.
Order of draw: after the EDTA tube and before the fluoride oxalate tube.
Each tube must be full.

Test Resources:

Submit requests using requisition or Connect Care online order entry:

Molecular Pathology Requisition

Please ensure all required information, including patient's clinical history/indication, is provided on the requisition.

Stability and Storage:

Room Temperature stable.
Sample must be received to the Mayo Clinic within 7 days of collection, therefore submission of the samples to the Molecular Pathology South Laboratory as soon as possible after collection is critical.

Processing:

Do not centrifuge.
Do not aliquot.
Do not refrigerate or freeze.
Keep specimens at room temperature until ready to transport.

Transportation:

Molecular Pathology North	Molecular Pathology South
Transport specimens to Molecular Pathology through UAH Specimen Receiving WMC 4B2.10. Molecular Pathology Staff Only Upon receiving specimens: Indicate primary EGFR mutation (ie. exon 19 deletion, L858R, etc.) on the requisition. Fax requisition to the Molecular Pathology Accessioning Desk at 403-944-4748. Forward the requisition and the Streck tubes to Molecular Pathology South.	Do not use pneumatic tube system for transport Transport to Anatomical Pathology Department Gross Room, 7th floor McCaig Tower, Foothills Medical Center.

Method:

Droplet Digital Polymerase Chain Reaction (PCR)

Method and Interpretation of Results:

Primary usage is for analysis and detection of EGFR T790M mutation and is performedas a send out test to Mayo Clinic.
Please refer to Mayo Clinic test directory page for addition information:
T790M - Overview: Cell-Free DNA EGFR T790M Mutation Analysis, Blood (mayocliniclabs.com)

Routine Turn Around Time:

10 days

Testing Schedule:

Weekly

Testing Area:

Molecular Pathology

Performing Site:

Foothills Medical Center

Contact Comments:

Molecular Pathology North	Molecular Pathology South
780-407-6648	403-220-4240

Last Updated On:

Friday, June 30, 2023

Date of Last Review:

Aug 1 2022 12:00AM

Registered Trademark Comments:

STRECK Cell-Free DNA BCT^® is a registered trademark of Streck, Inc.