Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

Thrombosis Investigations (Synonym: Protein S, Protein C, Antithrombin, Factor V Leiden, Prothrombin G2021A Variant, Antiphopholipid Syndrome Investigation)

Collection Alert:

Test cannot be collected by Mobile Collection Service.

Shipping and Handling Alert:

Specimens are time sensitive.

LABID (Connect Care):

LAB4347

Specimen Type:

Blood

Specimen Source:

Blood

Primary Container:

LT BLUE CITRATE and LAVENDER EDTA
If Antiphopholipid Syndrome Investigation is ordered, also GOLD SST.

Minimum Collection Volume:

For testing sent to Calgary:
Five 2.7 mL LT blue citrate tubes, allow the vacuum tube to completely fill, minimum fill is to frosted line on tube and one Lavender EDTA. If Antiphopholipid Syndrome Investigation is ordered also collect one GOLD SST minimum 3.0 mL.

For testing sent to Edmonton:
One 2.7mL LT blue citrate tube per test and one Lavender EDTA. If Antiphopholipid Syndrome Investigation is ordered also collect one GOLD SST minimum 1.0 mL and another GOLD SST minimum 3.0 mL.

For pediatric and neonate testing contact Special Coagulation Laboratory

Specimen Collection Requirements:

Light Blue Citrate: It is important to fill tube to maximum draw.
Mix well immediately after collection by inverting tube 3 to 4 times. If venous access is not available, sample can be drawn from a line but 6x the volume of the line or 5 mL (whichever is less) must be removed prior to collection. When using a winged blood collection set (butterfly needle), a discard tube must be used to remove the air in the tubing, if only a citrate tube is drawn or is the first tube drawn.

Test Resources:

Special Coagulation Testing Requisition Thrombosis Investigation

Stability and Storage:

Lt Blue Citrate: Room temperature – 3.5 hours

Processing:

For Gold SST:

Referred in specimens must be sent as frozen serum
Two 0.5 mL aliquots AND two 1.5 mL aliquots in plastic transport tubes
Label aliquots as “serum”

For LT BLUE CITRATE specimens:

For testing sent to Calgary:	For testing sent to Edmonton:
For specimens that will be received beyond stability: Centrifuge to obtain platelet poor plasma (platelet count <10 x 10*9/L) Aliquot plasma as per Special Coagulation Specimen Aliquoting	Centrifuge to obtain platelet poor plasma (platelet count <10 x 10*9/L) Aliquot each test as per Special Coagulation Aliquoting

For EDTA:

Room temperature, unspun

Transportation:

Room temperature – uncentrifuged
Frozen (dry ice if available)

Routine Turn Around Time:

Calgary:
For stat testing contact Special Coagulation Laboratory
After hours contact the Hematopathologist on call.
Edmonton:
For stat testing MUST contact the Hematopathologist on call.

Testing Schedule:

Batched

Reference Interval:

See Laboratory Report

Testing Area:

Special Coagulation

Performing Site:

Calgary Zone	APL Diagnostic and Scientific Centre
Edmonton Zone	University of Alberta Hospital

Contact Comments:

1-877-868-6848

Last Updated On:

Tuesday, August 1, 2023