Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

Chimerism Studies - Post Transplant, Recipient (Synonyms: Sorted Cell testing, Flow Sort Chimerism, Engraftment Studies - Post-Transplant)

Clinical Indications:

Investigation and monitoring of chimerism in post-bone marrow transplant patients.

Test Includes:

Analysis of 16 polymorphic chromosomal loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, AMEL, D5S818, FGA).

Ordering Alert:

Answer Order Questions by indicating the correct bone marrow transplant date.

LABID (Connect Care):

LAB4400

Specimen Type:

Blood, Bone Marrow

Primary Container:

See Specimen Collection Requirements

Specimen Collection Requirements:

For Blood specimen	For Adults: Collect one yellow top ACD "A" or one lavender top EDTA tube containing a minimum of 1.0 mL of blood. For Pediatrics: Collect one full pediatric blue top Sodium Citrate tube.
For Bone Marrow specimen	Collect 0.5 mL of bone marrow in one Flow Bone Marrow Medium (BMM) or EDTA.

Test Resources:

Submit request using the applicable requisition or Connect Care online order entry:

For Bone Marrow Collections		For Peripheral Blood Collections
	Bone Marrow Collections: Bone Marrow Pathology Requisition Complete Flow Sort section under Flow Cytometry – Bone Marrow.		Peripheral Blood Collections: Flow Cytometry Requisition Complete Cell Sorting and Chimerism Investigation section of the requisition.

Please ensure all required information, including patient's clinical history/indication, is provided on the requisition.

Stability and Storage:

Room temperature for 24 hours.
Refrigerate if storage is prolonged.

Processing:

Do not centrifuge or aliquot.
Do not freeze.

Transportation:

Transport specimens at room temperature to Foothills Medical Center Flow Cytometry within 24 hours of collection.

Method:

Multiplex PCR followed by capillary electrophoresis.

Method and Interpretation of Results:

Chimerism status is determined by amplification of short tandem tetranucleotide repeats with a panel of fluorochrome labelled primers at 16 chromosomal loci using the AmpFlSTR Identifiler kit from Applied Biosystems (ABI). Amplified products are detected using the ABI 3500DX genetic analyzer. Pre-transplant recipient and donor allele patterns are compared to post-transplant recipient allele patterns. ABI Genemapper Software is used to determine the relative donor allele percentage.

Test sensitivity for detecting mixed chimerism by this method is routinely 3-5% minor allele as determined using in-house validation with defined mixtures of DNA.

Routine Turn Around Time:

2 weeks

Testing Schedule:

Batched

Testing Area:

Molecular Pathology South (DSC)

Performing Site:

Diagnostic and Scientific Centre

Additional Test Information:

To obtain Bone Marrow Medium (BMM) tube, contact:

Flow Cytometry

403-944-4771

Hematology Translational Lab will sort post-transplant cells and forward to Molecular Pathology South (DSC) for chimerism testing.

Contact Comments:

Molecular Pathology South (DSC) 403-770–3699

Last Updated On:

Wednesday, February 21, 2024

Date of Last Review:

Feb 8 2024 12:00AM