Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

Neonatal Evaluation, Cord (Cord ABORH, Neonatal ABORH, Fetal-Maternal Investigation, NEV, RhIG Evaluation, Postnatal)

Clinical Indications:

Determination of maternal post-partum eligibility for RhIG
To aid in the diagnosis of hemolytic disease of the newborn (HDN)

Test Includes:

Testing protocol may include:
ABO Type
Rh Type
Direct Antiglobulin Test (DAT)
Antibody screen and identification (eluate)
Antigen typing

Ordering Alert:

Testing must be ordered on the neonate.

Ensure mother’s identification information is provided.

For maternal postnatal investigation, see Postnatal Evaluation (Mother)

Collection Alert:

Testing should be performed on cord blood, if available.

If cord blood is not available, ABORH and Direct Antiglobulin Test (DAT) may be requested on neonatal whole blood. Contact your laboratory for ordering instructions.

LABID (Connect Care):

LAB892

Test Code:

CALGARY ZONE: NEWBORN CORD SPECIMEN
CENTRAL ZONE, SOUTH ZONE: CORD
NORTH ZONE:PNN OR PNNRO1

Specimen Type:

Blood

Specimen Source:

Cord Blood

Primary Container:

Lavender top - EDTA

Alternate Containers:

Central Zone, Edmonton Zone: Red top – no additive
Calgary Zone, North Zone, South Zone: No alternate

Minimum Collection Volume:

1 mL

Test Resources:

Requisitions:
Calgary Zone: Postnatal Requisition
Central Zone, North Zone, South Zone: APL Transfusion Medicine Requisition
Edmonton Zone: Regional Blood Transfusion Service Requisition

Resources for Midwives:
Edmonton Zone: Maternal and Cord Specimen Handling
North Zone: Midwife Postpartum and Cord Blood Specimen Handling (Grande Prairie)

Stability and Storage:

Refrigerated 1 - 6 °C
Stored 7 days

Method and Interpretation of Results:

An Rh positive neonate born to an Rh Negative mother indicates the mother is eligible for RhIG, unless the mother has an immune anti-D.

A positive DAT may indicate maternal antibody is bound to neonatal red blood cells. A positive DAT may also occur due to recent maternal RhIg injection.

Antibody identification testing and maternal history/testing are used to determine whether a positive DAT is due to a maternal antibody implicated in hemolytic disease of the newborn (HDN).

Testing Schedule:

Daily

Testing Area:

Transfusion Medicine

Performing Site:

Calgary Zone	Foothills Medical Centre
Central Zone	Camrose General Hospital Drumheller HC Red Deer Regional Hospital Centre Wainwright HC Wetaskiwin Hospital
Edmonton Zone	Grey Nuns Hospital Misericordia Hospital Royal Alex Hospital Sturgeon Hospital
North Zone	Bonnyville HC Cold Lake HC Edson HC Grande Prairie Regional Hospital High Prairie HC Hinton HC Lac La Biche HC Peace River HC St. Paul HC
South Zone	Chinook Regional Hospital Medicine Hat Regional Hospital

Last Updated On:

Tuesday, August 11, 2020