Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

Cytogenetic Analysis, Constitutional (Synonyms: Chromosome Analysis, Cytogenetic testing, Cytogenetics, G-Band (GTG) Analysis, Karyotype, Fluorescence In Situ Hybridization, FISH)

Collection Alert:

Unopened dedicated tubes and containers (do not share) are required for Cytogenetic analysis.

LABID (Connect Care):

LAB8882

Specimen Type:

Amniotic Fluid, Blood, Other Body Fluid, Pleural Fluid, Tissue, Tissue – Fetal, Urine - Fetal

Specimen Source:

Indicate a specimen source.

Primary Container:

See Specimen Collection Requirements.

Minimum Collection Volume:

If unable to collect the minimum as per specimen collection requirements, contact Cytogenetics.

Specimen Collection Requirements:

Amniotic or Other Fluid:

Submit 25.0 to 30.0 mL of fluid in an appropriate sized sterile polypropylene centrifuge tube.

Chorionic Villi Sample:

Submit 30.0 mg in sterile polypropylene centrifuge tube containing CVS transport media.
If submitting to Cytogenetics North: CVS is received Monday to Thursday, 08:00 to 15:00 only. Cannot be collected to be received on Fridays.

Blood Specimens:
For Karyotype or FISH

Newborn to 6 months: Collect one green top Sodium Heparin (no gel) containing 1.0 to 3.0 mL of blood.
6 months to Adult: Collect one green top Sodium Heparin (no gel) containing a 3.0 to 5.0 mL of blood.

Skin Biopsy Specimen:
For Karyotype

Submit a skin biopsy in a sterile container containing RPMI or transport media.
DO NOT put tissue in formalin.

Test Resources:

Pre-test Counselling Information-Genetic and Genomic Testing

Submit requests using requisition or Connect Care online order entry:

Cytogenetic Analysis, Constitutional Requisition

Please ensure all required information, including patient's clinical history/indication, is provided on the requisition.

If submitting tissue specimens from multiple specimen sources on a patient, submit each specimen, in a separate specimen bag with a separate requisition.

If a Chromosomal Microarray Analysis (CMA) will be also be requested, please refer to Cytogenetic Analysis, Microarray test directory page for specimen requirements.

Stability and Storage:

Room temperature up to 24 hours: amniotic or other fluid, chorionic villi
Room temperature up to 7 days: blood
Refrigerated up to 48 hours: fresh tissue

Processing:

Do not centrifuge, aliquot or separate specimens.
Do not put tissue specimens in formalin.
Maintain specimens at appropriate storage temperature until transported.

Transportation:

ALL specimens should be received in the laboratory on the day of collection (preferred) or within 24 hours of time of collection.

Submit to Cytogenetics North	Submit to Cytogenetics South
Specimens collected outside of regular hours of Monday to Friday (08:00 to 16:00) should be shipped to UAH Specimen Control, WMC 4B2.10.	When submitting Amnio or CVS Call Cytogenetics at 403-955-2886 to notify that the specimen is being transported.
Transport at room temperature to Cytogenetics Laboratory via UAH Specimen Control WMC 4B2.10.	Transport at room temperature to the ACH Cytogenetics Laboratory B0-150.

Routine Turn Around Time:

Blood	Routine 4 weeks, Stat 1 week
Amniotic Fluid	2 weeks
Chorionic Villi	3 weeks
Tissue	6 weeks

Testing Schedule:

Daily

Reference Interval:

Interpretation provided on report.

Testing Area:

Cytogenetics

Performing Site:

University of Alberta Hospital or Alberta Children's Hospital

Additional Test Information:

Amniotic Fluid - Karyotype
If RAD testing is abnormal, karyotype testing will then be added on as a reflex test.

If a sample is received with indicating a family history of chromosomal abnormality or follow up chromosomal analysis, karyotype testing may be performed at the discretion of the Cytogeneticist.

Chorionic Villi

Cytogenetics North	Cytogenetics South
The collection of the chorionic villi is only performed at the Maternal Fetal Medicine Clinic, Royal Alexandra Hospital in Edmonton.	The collection of the chorionic villi is only performed at the Maternal Fetal Medicine Center, EFW Radiology in Calgary.

Chorionic Villi - Karyotype
Chorionic Villi will also include RAD testing and karyotype testing, with the exception that karyotyping will NOT be performed on CVS specimens collected for single gene testing.

Other Information

Cytogenetics North	Cytogenetics South
To order CVS transport media, RPMI or tissue transport media tubes call Cytogenetics North 780-407-1542.	To order CVS transport media, RPMI or tissue transport media tubes call Cytogenetics South 403-955-7375.

Contact Comments:

For additional information refer to Genetics and Genomics webpage or contact:

Cytogenetics North	Cytogenetics South
780-407-1542 (phone) 780-407-3059 (fax)	403-955-7375 (phone) 403-955-3000 (fax)

Last Updated On:

Monday, April 1, 2024

Date of Last Review:

Aug 1 2022 12:00AM