Alberta Precision Laboratories

--- Query Results ---

Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

Prothrombin G20210A Mutation (Synonyms: Factor II G20210A Variant, THROMB, Factor II Polymorphism, F2MUT)

Clinical Indications:

Investigation of thrombophilia

Ordering Alert:

Submitting to Molecular Pathology North: Order Thrombosis Investigations

Submitting to Molecular Pathology South DSC: Order Thrombosis Investigations

Collection Alert:

Calgary Zone: Test cannot by collected by Mobile Collection Service.

Unopened dedicated tubes are required for Molecular testing.

LABID (Connect Care):

LAB834

Test Code:

EDMONTON ZONE	LAB834
CALGARY ZONE	LAB4347

Specimen Type:

Blood

Primary Container:

See Specimen Collection Requirements

Specimen Collection Requirements:

Molecular Pathology North	Molecular Pathology South DSC
Refer to Thrombosis Investigations	Refer to Thrombosis Investigations

Test Resources:

Submit request using the applicable requisition or Connect Care online order entry:

Molecular Pathology North		Molecular Pathology South DSC
	Refer to Thrombosis Investigations to obtain requisition.
			Refer to Thrombosis Investigations to obtain requisition.

Please ensure all required information, including patient's clinical history/indication, is provided on the requisition.

Stability and Storage:

Refrigerated up to 3 days

Processing:

Molecular Pathology North	Molecular Pathology South DSC
Refer to Thrombosis Investigations	Refer to Thrombosis Investigations

Transportation:

Molecular Pathology North	Molecular Pathology South DSC
Refer to Thrombosis Investigations	Refer to Thrombosis Investigations

Method:

Molecular Pathology North
Real-time PCR

Molecular Pathology South DSC
Hemi-Nested Allele Specific PCR, followed by electrophoresis.

Method and Interpretation of Results:

Molecular Pathology North
After DNA isolation from a peripheral blood sample, polymerase chain reaction using a custom TaqMan Genotying assay (Thermo Fisher Scientific, USA) was used. This assay uses fluorescently-labeled probes to detect the presence of a single point mutation (G to A at position 20210) of the human Factor II gene.

Molecular Pathology South DSC
Diluted whole blood or DNA isolated from peripheral blood is used to amplify a single point mutation (G20210A) located in the 3' non-coding region of the prothrombin gene by hemi-nested pcr. Allele specific amplified products are visualized using the Agilent 4150 TapeStation^®. Interpretation is based upon the presence of an additional band in the A-allele specific reaction that corresponds to the expected product size of the 20210A mutation.

Routine Turn Around Time:

Molecular Pathology North	Molecular Pathology South DSC
1 month	Refer to Thrombosis Investigations

Testing Schedule:

Batched

Testing Area:

Molecular Pathology

Performing Site:

University of Alberta Hospital or Diagnostic and Scientific Center

Contact Comments:

Molecular Pathology North	Molecular Pathology South DSC
780-407-6648	403-770-3699

Last Updated On:

Wednesday, February 28, 2024

Date of Last Review:

Feb 22 2024 12:00AM

Registered Trademark Comments:

TapeStation^® is a registered trademark of Agilent Technologies.