Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

Non-Gynecological Cytology; (Synonyms: Sputum (Malignant Cells) Cytology, Non-Gyne Cytology, Non-Gynecological, Cytology)

Pathogen:

Malignancy

Clinical Indications:

This test is performed to diagnose malignancy in sputum

LABID (Connect Care):

LAB13

Test Code:

CALGARY ZONE, CENTRAL, EDMONTON, AND NORTH ZONES	NOT SPECIFIED
SOUTH ZONE	CYSP

Specimen Type:

Fresh Sputum

Specimen Source:

Sputum

Primary Container:

Sterile container
Central Zone: Refer to DynaLIFE. Red Deer Regional Hospital – Sterile container.

North Zone: Refer to DynaLIFE

Specimen Collection Requirements:

Early morning deep cough specimens are the most sensitive. Patients should clean teeth and mouth prior to obtaining specimen. Instruct patient to take a deep breath and cough in order to procure a deep cough specimen. If non-productive specimens are obtained, induced specimens may be required.

Calgary Zone: Submit fresh sputum (no fixative). A series of three early morning deep cough specimens is the most sensitive.

Central Zone: A separate specimen is required. DO NOT combine with AFB/TB. Refer to DynaLIFE.
Red Deer Regional Hospital - Submit fresh sputum. No fixative. A separate specimen is required. DO NOT combine with AFT/TB.

Edmonton Zone: Submit fresh sputum (no fixative).

North Zone: A separate specimen is required. DO NOT combine with AFB/TB. Refer to DynaLIFE.

South Zone: Submit fresh sputum (no fixative). A separate specimen is required. DO NOT combine with AFB/TB.

Containers must be securely tightened to avoid leakage.
Place collected specimen in a sealed biohazard bag.
Place requisition in outside pouch.

For sites referring specimens to DynaLIFE, see Sputum cytology in the DynaLIFE Medical Labs Test Directory or contact DynaLIFE Specimen Control at 1-800-661-9876.

Test Resources:

Calgary Non-Gynecological Cytopathology Requisition

Sputum for Cytopathology Patient Instructions

Central Non-Gynecological Cytology Requisition

Edmonton Nongynecologic Cytopathology Requisition

South Non-Gynecological Cytology Request

Stability and Storage:

Room temperature up to 4 hours
Refrigerate after 4 hours

Transportation:

It is preferable that a separate sputum specimen be collected for each testing area (e.g. Microbiology and Cytopathology) to ensure each area receives an adequate sample. However, if only 1 sample is received and Microbiology testing is required, the specimen must be sent there first.

Calgary Zone: If tests other than Cytopathology are required on the specimen, a requisition for each department must be completed and accompany the specimen.
Edmonton Zone: University of Alberta Hospital only:
Deliver to the Anatomical Pathology Laboratory (Specimen Receiving) Walter Mackenzie Centre (WMC) 5B4.29 immediately.
After hours and weekends are to be delivered to Laboratory Medicine and Pathology (Specimen Control) WMC 4B2.10.
South Zone: Have patient deliver specimen to laboratory on same day of collection.

Method:

Liquid Based Cytology (LBC)

Routine Turn Around Time:

Within 3 working days

Testing Schedule:

Monday to Friday

Testing Area:

Cytopathology

Performing Site:

Calgary Zone	Diagnostic and Scientific Centre
Central Zone	Red Deer Regional Hospital DynaLIFE Medical Labs
Edmonton Zone	University of Alberta Hospital DynaLIFE Medical Labs
North Zone	DynaLIFE Medical Labs
South Zone	Chinook Regional Hospital

Additional Test Information:

Submission of a complete sputum series is preferable as it increases diagnostic accuracy. Collect a "deep cough" early morning sputum sample each day for 3 consecutive days.

A post-bronchoscopy sputum specimen, collected immediately following the bronchoscopy, is an extremely valuable specimen since cancer cells desquamate following bronchoscopy. Two more early morning specimens should then be collected on the next 2 days thereby completing the post-bronchoscopy series.

Last Updated On:

Friday, December 23, 2022