Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

Non-Gynecological Cytology; (Synonyms: Fine Needle Aspirate (Malignant Cells) Cytology, FNA, Aspirate, Cyst Fluid, FNA Slide, CT/Ultrasound Guided FNA, Palpable Lesions, Deep Seated Lesions, Endoscopic Ultrasound-Aspirate EUS)

Pathogen:

Malignancy

Clinical Indications:

Neoplastic and non-neoplastic mass lesions: benign neoplasms, inflammatory/infections, abscesses, malignant neoplasms

Collection Alert:

Calgary Zone and Edmonton Zone: If Lymphoma is suspected, an additional specimen collected in TTM (Tissue Transport Media) is required for Flow Cytometry.

Calgary Zone: To order collection tubes of TTM, refer to Request for Tissue and Bone Marrow Transport Media - Flow Cytometry

LABID (Connect Care):

LAB13

Test Code:

CALGARY, CENTRAL, EDMONTON AND NORTH ZONES	NOT SPECIFIED
SOUTH ZONE	CYFNA, CYBR, CYTH

Specimen Type:

Aspirate

Specimen Source:

Superficial Palpable Lesions, Deep Seated Lesions

Primary Container:

Calgary Zone	CytoLyt® container
Central Zone	Refer to DynaLIFE for FNA. For EUS - 10 mL LH Diluent and appropriate sized container with 10% Neutral Buffered formalin. Red Deer Regional Hospital - Sterile container
Edmonton Zone	Glass slides (2)
North Zone	Refer to DynaLIFE for FNA. For EUS - 10 mL LH Diluent and appropriate sized container with 10% Neutral Buffered formalin
South Zone	30 mL plastic container filled with 7.5 mL CytoLyt® solution

Alternate Containers:

Glass slide
Edmonton Zone: Screw top tube or sterile container

Specimen Collection Requirements:

Calgary Zone: Expel the contents of the syringe into a CytoLyt^® container and rinse the needle in the same container. For optimal sampling, use a 20-22 gauge needle and aspirate the lesion using multiple passes.

Central Zone: For EUS: Expel the aspirate into 10 mL LH Diluent and appropriate sized container with 10% Neutral Buffered formalin.

Red Deer Regional Hospital - Expel the contents of the syringe into a container of sterile saline. Rinse the needle in the same container.

Edmonton Zone: After each pass, express specimen on slide, gently smear the material to create an air dried and an alcohol fixed slide. Alcohol fixation must happen immediately to prevent air-dry artifact. Submit 2 air dried slides and 2 alcohol fixed slides. For any additional passes, express into formalin in either 10 mL screw top tube or 90 mL sterile container so that a cell block may be prepared. Submit the entire aspiration for optimal cytologic evaluation. If an infection is suspected, material should be sent directly to the microbiology laboratory. If the aspirate yields cyst fluid, the fluid may be expressed into a sterile tube, unfixed for cytospin preparation. It is recommended that a second pass be performed from any remaining solid component to ensure adequate sampling of the lesion.

North Zone: For EUS: Expel the aspirate into 10 mL LH Diluent and appropriate sized container with 10% Neutral Buffered formalin.

South Zone: Expel sample and rinse needle directly into a container pre-filled with CytoLyt^®solution. Two passes are preferred.

Containers must be securely tightened to avoid leakage.
Place collected specimen in a sealed biohazard bag.
Place requisition in outside pouch.

For sites referring specimens to DynaLIFE, see Fine needle aspirate cytology in the DynaLIFE Medical Labs Test Directory or contact DynaLIFE Specimen Control at 1-800-661-9876.

Test Resources:

Calgary Non-Gynecological Cytopathology Requisition

Central Non-Gynecological Cytology Requisition

Edmonton Nongynecologic Cytopathology Requisition

South Non-Gynecological Cytology Request

Stability and Storage:

Refrigerate specimen if a delay in transport is anticipated

Transportation:

Calgary Zone: This procedure may yield an aspirate and a needle biopsy. Place the needle biopsy in a small formalin container. Send both samples to Cytopathology. If only a needle biopsy is collected, send the formalin container to Anatomic Pathology with Anatomical Pathology Requisition (Consultation Request).

Edmonton Zone: Deliver to Anatomical Pathology Laboratory (Specimen Receiving) Walter Mackenzie Centre (WMC) 5B4.29 immediately.

Method:

Liquid Based Cytology (LBC), Smear, Cytospin, Cell block

Routine Turn Around Time:

Within 3 working days

Testing Schedule:

Monday to Friday

Testing Area:

Cytopathology, Anatomic Pathology

Performing Site:

Calgary Zone	Diagnostic and Scientific Centre
Central Zone	Red Deer Regional Hospital DynaLIFE Medical Labs
Edmonton Zone	University of Alberta Hospital
North Zone	Grande Prairie QE II Regional Hospital DynaLIFE Medical Labs
South Zone	Chinook Regional Hospital

Additional Test Information:

Central Zone:
For EUS: pre-booking is required when possible; Pathologist and Technologist required to be present for this procedure.

Red Deer Regional Hospital: In-Hospital Fine Needle Aspiration Procedure. The Cytotechnologist will attend FNA, prepare the slides and assess sample for adequacy.

North Zone: For EUS: pre-booking is required when possible; Pathologist and Technologist required to be present for this procedure.

Last Updated On:

Friday, December 23, 2022

Registered Trademark Comments:

CytoLyt^® is a registered trademark of Hologic