Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

Leukemia/Lymphoma Immunophenotyping, Blood (Synonym: FACS, Flow Cytometry, HEMFLOW, LEUK/LOMA, MRD, XFACS)

Clinical Indications:

Flow based immunophenotypic testing represents an accurate and invaluable tool for the clinician and the pathologist in the diagnosis, subclassification, prognostication and follow up (MRD) of acute and chronic leukemias as well as non-Hodgkin lymphomas.

Clinical Indications may include:

Acute Leukemia
Lymphoma
Lymphoproliferative Disorder
Minimal Residual Disease
MRD - AML
MRD - B-ALL
MRD - T-ALL
Pancytopenia (bone marrow only)
Myelodysplastic Syndrome (MDS) (bone marrow only)
Plasma Cell Dyscrasia
Plasma Cell Neoplasm

Test Includes:

Immunophenotyping

Ordering Alert:

CBC with Differential must be ordered for the same specimen collection time.

This information is for blood specimens only. For other specimen types, see Leukemia/Lymphoma Immunophenotyping, Non-Blood

Shipping and Handling Alert:

Specimens must be delivered within 24 hours of collection

For testing sent to Edmonton: specimens must be delivered by 12:00 noon on the day before a weekend or statutory holiday.

LABID (Connect Care):

LAB4328

Test Code:

NORTH ZONE	ASPEN: FLOWBLD
SOUTH ZONE	LETHBRIDGE AND AREA: FLOWBLD MEDICINE HAT AND AREA: FLOWBLD2

Specimen Type:

Blood

Primary Container:

Calgary: PALE YELLOW (ACD-A)
Edmonton: GREEN NA HEP and LAVENDER EDTA

Alternate Containers:

Calgary: LAVENDER EDTA, GREEN NA HEP, LT BLUE CITRATE

Specimen Collection Requirements:

Hemolyzed, frozen, or clotted specimens are not accepted.

For testing sent to Calgary:
Adult: Collect in 8.5 mL PALE YELLOW (ACD-A) tube (full tube is required). Pediatric: Minimum 1.8 mL LT BLUE CITRATE tube

For testing sent to Edmonton:
Collect minimum 1 mL in GREEN NA HEP and LAVENDER EDTA tubes.

Test Resources:

For testing sent to Calgary:		For testing sent to Edmonton:
	Flow Cytometry - FC3200 (Calgary) Flow Cytometry Out of Province Requisition - FC3200EP (Calgary)		Hematology Specialty Requisition RPASPF00002UAH Referred In Specimen Tracking Form

Stability and Storage:

GREEN NA HEP: 48 hours
LAVENDER EDTA: 24 hours
PALE YELLOW (ACD-A): 5 days

Processing:

DO NOT centrifuge.

For testing sent to Calgary from outside Calgary Zone and testing sent to Edmonton: Send 1 stained or unstained blood smear along with collection tube.

Transportation:

For testing sent to Calgary from outside Calgary Zone:

Transport at refrigerated temperature
Transport by FedEx overnight shipping. Fax the tracking number and requisition to 403-270-4135.
Ship directly to:
Flow Cytometry Laboratory
7th Floor McCaig Tower, Foothills Medical Centre
1403 - 29 Street NW, Calgary, AB, Canada T2N 2T9

For testing sent to Edmonton from outside Alberta:

Transport at room temperature
Complete Referred In Specimen Tracking Form, provide expected arrival date and time, check priority “Time Sensitive”, and fax to 780-407-6267.
Ship directly to:
Specimen Control, Laboratory Medicine and Pathology
4th Floor Walter Mackenzie Centre (4B2.10)
University of Alberta Hospital
8440 112 Street NW, Edmonton, AB, Canada T6G 2B7

Testing Schedule:

Monday to Saturday

Reference Interval:

See laboratory report

Testing Area:

Flow Cytometry

Performing Site:

Calgary: Foothills Medical Centre
Edmonton: University of Alberta Hospital

Additional Test Information:

Requisition and CBC with Differential results must accompany the specimen if unavailable in Netcare.

Patient history or indication for testing is required.

For testing sent to Calgary from within the Calgary Zone: tests ordered by non-Hematologists will be screened using current CBC, Differential and smear results. Testing will only be done when one or more of the following criteria are met AND testing has not been done within the last 3 months.

Lymphocytosis greater than 3.0 x 10⁹/L
Monocytosis greater than 1.0 x 10⁹/L
Thrombocytopenia less than 100 x 10⁹/L
Blasts present
Flow Cytometry testing recommended from a previous peripheral blood smear report

Contact Comments:

1-877-868-6848

Last Updated On:

Monday, June 26, 2023