Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

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Test Name/Synonym:

Testosterone, Free (Synonym: FTEST, TTF)

Test Includes:

Free Testosterone is a calculation involving measurement of Total Testosterone, Albumin, and Sex Hormone Binding Globulin.

Collection Alert:

Calgary: A serum specimen is required for Testosterone, Free.

LABID (Connect Care):

LAB173

Specimen Type:

Blood

Primary Container:

Calgary: Two GOLD SST tubes
Edmonton: GOLD SST

Alternate Containers:

Calgary: RST and MINT LIHEP PST
Edmonton: MINT LIHEP PST

Minimum Collection Volume:

Calgary: 0.5 mL serum or plasma in each tube
Edmonton: 1 mL serum or plasma

Test Resources:

General Laboratory Requisition – 21302 – write test name under “Additional Tests”

Calgary Zone: Microcollection Minimum Whole Blood Volumes

Calgary Zone: Acceptable Tests for Rapid Serum Tube (RST) Collection

Processing:

Edmonton: Centrifuge, aliquot and freeze serum or plasma.

Transportation:

Calgary: Room temperature
Edmonton: Frozen

Testing Schedule:

Monday to Friday

Reference Interval:

Age	Gender	pmol/L
18 years and greater
	Female	2 - 30
	Male	175 - 700

Reference intervals were established on specimens collected in the morning (07:00 - 10:00), therefore it is suggested to collect specimens within this timeframe.

Testing Area:

Biochemistry

Performing Site:

Calgary: Diagnostic and Scientific Centre
Edmonton: University of Alberta Hospital

Additional Test Information:

This test is performed by Calgary Immunochemistry and Edmonton Special Chemistry.

The concentration of Free Testosterone is derived from a mathematical expression based on constants for the binding of Testosterone to Sex Hormone Binding Globulin and Albumin.

Testosterone exhibits diurnal variation in adult males.
The amplitude of diurnal variation decreases with age, with patients aged greater than 70 years showing an approximate 10% decrease.
Diurnal variation of testosterone in females is not well defined in the literature, but similar variation may be expected in pre-menopausal women.

Reference:
Brambilla, DJ, Matsumoto, AM, Araujo, AB, McKinlay, JB. The Effect of Diurnal Variation on Clinical Measurement of Serum Testosterone and Other Sex Hormone Levels in Men. J. Clin. Endocrinol. Metab. 2009. 94(3):907-913

Last Updated On:

Thursday, April 4, 2024