Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

Immunodeficiency Screening Panel (Synonym: Flow Lymphocyte Subset, IDEF)

Test Includes:

Relative and absolute counts are reported for the following lymphocyte subsets: CD3, CD3+CD4+, CD3+CD8+, CD3+CD4-CD8-, CD3-CD16/56+, CD3+CD16/56+, CD19, CD19+IgD+CD27- (naïve), CD19+ IgD+CD27+ (memory non-class switched), CD19+IgD-CD27+ (memory class-switched). CD3+CD4+ and CD3+CD8+ subsets are further classified as naïve, terminally differentiated, central memory, and effector memory subsets using CD45RA and CD27.

Ordering Alert:

Calgary Zone: CBC with Differential must be ordered for the same specimen collection time.

Outside Calgary Zone: Send a copy of CBC with Differential report with specimen.

LABID (Connect Care):

LAB4464

Test Code:

CALGARY ZONE	IDEF
CENTRAL ZONE	MISC TO CLS
NORTH ZONE	ASPEN: FLOWBLD PEACE: FLOWOTHER
SOUTH ZONE	LETHBRIDGE AND AREA: FLOWBLD MEDICINE HAT AND AREA: MISC

Specimen Type:

Blood

Primary Container:

Adult: PALE YELLOW (ACD-A)
Pediatric: LT BLUE CITRATE
Neonates: MICROCOLLECTION EDTA

Alternate Containers:

LAVENDER EDTA, GREEN NA HEP, LT BLUE CITRATE

Minimum Collection Volume:

Adult and Pediatric: 0.5 mL
Neonates: 200 µL, if CBC already ordered otherwise 0.5 mL

Specimen Collection Requirements:

Adult: 8.5 mL PALE YELLOW (ACD-A)
Pediatric: 1.8 mL LT BLUE CITRATE
Neonates: 200 µL Micro-EDTA One MICROCOLLECTION EDTA

Test Resources:

Flow Cytometry – FC3200 (Calgary)

Flow Cytometry Out of Province Requisition – FC3200EP

Stability and Storage:

PALE YELLOW (ACD-A): 5 days
GREEN NA HEP: 48 hours
LT BLUE CITRATE: 48 hours
LAVENDER EDTA: 24 hours

Processing:

DO NOT centrifuge or freeze specimen.

Transportation:

Within Calgary Zone: transport at room temperature.

For testing sent to Calgary from outside Calgary Zone:

Transport at refrigerated temperature by FedEx overnight shipping. Specimen must be received within 24 hours of collection. Fax the tracking number and requisition to 403-270-4135.
Ship directly to:
Flow Cytometry Laboratory
7th Floor McCaig Tower, Foothills Medical Centre
1403 - 29 Street NW, Calgary, AB, Canada T2N 2T9

Method:

Flow Cytometry

Testing Schedule:

Monday to Friday

Reference Interval:

See laboratory report

Testing Area:

Flow Cytometry

Performing Site:

Calgary: Foothills Medical Centre

Additional Test Information:

Requisition and CBC with Differential results must accompany the specimen if unavailable in Netcare.

Patient history must be included on the requisition

Contact Comments:

1-877-868-6848

Last Updated On:

Friday, March 12, 2021