Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

Infliximab (Synonym:Inflectra, Remicade, Renflexis, anti-TNF inhibitor, infliximab level)

Clinical Indications:

This test is used to monitor patients taking Infliximab for inflammatory bowel disease.

Test Includes:

Infliximab will be performed on all specimens. Infliximab total antibody results are reported as a reflex order when infliximab trough level is less than 3 µg/mL; otherwise, order requires on-call clinical biochemist consultation with specific clinical indication.

Ordering Alert:

This test is restricted to gastroenterologists and pediatricians.

Connect Care users must answer the question on clinical indication.
For Non-Connect Care users EITHER a completed Infliximab Level Order Form with a general laboratory requisition OR an e-Clinician requisition must accompany specimen.

Collection Alert:

Draw blood immediately before next scheduled dose (trough specimen)

LABID (Connect Care):

LAB4630

Specimen Type:

Blood

Specimen Source:

Venous

Primary Container:

GOLD SST or RED

Alternate Containers:

LAVENDER EDTA

Minimum Collection Volume:

1 mL serum (preferred) or plasma

Test Resources:

General Laboratory Requisition – 21302

Infliximab Level Order Form

Stability and Storage:

Refrigerated and uncentrifuged: stable 6 hours
Refrigerated and centrifuged: stable 24 hours.
Store frozen (-20°C) for 2 months.

Processing:

Centrifuge and aliquot 0.5 mL serum or plasma into two polypropylene screw top aliquot tubes.

For pediatric specimens, 0.25 mL is acceptable.
Freeze aliquot tubes before transporting to University of Alberta Hospital.

Transportation:

Transport frozen to University of Alberta Hospital

Method:

Enzyme linked immunosorbent assay (ELISA)

Routine Turn Around Time:

Within 3 days (infliximab level)
Within 1 week (infliximab total antibody)

Testing Schedule:

Run infliximab level 3 times a week

Reference Interval:

Test	Reference Interval	Comment	Units
Infliximab level (Induction Trough)	0.4 – 45.0		µg/mL
Infliximab level (Steady State)	3.0 - 7.0	Therapeutic
	Less than 3.0	Sub-therapeutic		µg/mL
Infliximab total antibodies	Less than 25	Undetectable	AU/mL
Infliximab total antibodies	25 - 492	Detectable	AU/mL

Infliximab pediatric reference intervals are not established.

Testing Area:

Biochemistry

Performing Site:

Edmonton Zone: University of Alberta Hospital

Additional Test Information:

This test is performed by Edmonton Special Chemistry.

For urgent requests, contact Client Response at 780-407-7484 for the clinical Biochemist on call information.

Last Updated On:

Monday, December 11, 2023