Alberta Precision Laboratories

--- Query Results ---

Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

HLA Antibody Screening

Ordering Alert:

If a paper requisition is used to order the test, the following information MUST be included:

CRITICAL (Samples cannot be processed if this information is not provided)

What organs are being considered?
What program is the patient followed by? (Calgary/Edmonton)

IMPORTANT (Any omissions can result in significant delays in testing)

New Transplant work up?
- If Yes: Previous transplants?
- If No, indicate:
  - Pre-transplant testing? If yes:
    - Transfused since last HLA antibody testing?
  - Post- transplant testing? If yes, provide indication:
- Graft Dysfunction – biopsy proven
- Graft Dysfunction – biopsy pending
- Known DSA
- Post transplant Monitoring
- Routine Scheduled Biopsy
- Store Only
Calgary: For urgent testing contact the Transplant Laboratory South at 403-770-3652.

Edmonton: For urgent testing contact the Transplant Laboratory North at 780-407-8881. After hours, page the HLA On-Call Technologist through Locating.

If a paper requisition is used for order entry into Connect Care, the requisition MUST accompany the specimen to the performing laboratory.

LABID (Connect Care):

LAB6057

Specimen Type:

Blood

Primary Container:

RED

Alternate Containers:

GOLD SST

Minimum Collection Volume:

7 mL
Pediatric patients: contact performing laboratory for minimum requirements

Specimen Collection Requirements:

ECMO patients, collect GREEN NA HEP or MINT LIHEP PST.

Test Resources:

For testing sent to Calgary:		For testing sent to Edmonton:
	Histocompatibility and Immunogenetics (HIL)/Tissue Typing Requisition, Solid Organ Transplant, HIL - REQ9054TT-SO (Calgary)		Histocompatibility Requisition - CH-0319 (Edmonton)

Processing:

For testing sent to Calgary:

Centrifuge and aliquot the entire volume of serum from the HLA Antibody Screening test tube(s).
If specimen will not be transported within 24 hours of collection, store serum frozen until shipment.

For testing sent to Edmonton:

If specimen will be transported within 24 hours of collection, no processing is required.
If specimen will not be transported within 24 hours of collection, centrifuge and aliquot the entire volume of serum or plasma from the HLA Antibody Screening test tube(s).

Transportation:

For testing sent to Calgary:

Transport serum aliquots at refrigerated temperature.
Transport previously frozen aliquots frozen.
If collection site is unable to centrifuge specimen, transport uncentrifuged (whole blood) specimen at room temperature.
Diagnostic Scientific Centre: Deliver serum aliquots directly to HIL. After 15:00, place serum aliquots in HIL -20ºC freezer.

For testing sent to Edmonton:

Transport whole blood at room temperature.
Transport serum or plasma at refrigerated temperature.

Testing Schedule:

Monday to Friday

Testing Area:

Transplant Laboratory

Performing Site:

Calgary: Diagnostic and Scientific Centre
Edmonton: University of Alberta Hospital

Contact Comments:

Calgary: Transplant Laboratory South at 403-770-3652.
Edmonton: Transplant Laboratory North at 780-407-8881.

Last Updated On:

Wednesday, April 24, 2024