Alberta Precision Laboratories

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Organization:

Alberta Precision Laboratories

Message Alert:

PLEASE NOTE: This Test Directory is in an interim state and due to changes pertaining to the DynaLIFE transition, performing site for routing has not been updated. Please refer to Laboratory Information System for appropriate routing.

Test Name/Synonym:

Antiphospholipid Syndrome Investigation
(Synonym: Anti-Beta-2 Glycoprotein, Anti-Cardiolipin Antibody, Anticardiolipin, APA, Cardiolipin, DRVVT, Dilute Russell Viper Venom Time, Lupus Anticoagulant)

Collection Alert:

Test cannot be collected by Mobile Collection Service.

Shipping and Handling Alert:

Specimens are time sensitive.

LABID (Connect Care):

LAB478

Specimen Type:

Blood

Specimen Source:

Blood

Primary Container:

LT BLUE CITRATE and Gold SST

Minimum Collection Volume:

For testing sent to Calgary:
Three 2.7 mL LT blue citrate tube, allow the vacuum tube to completely fill, minimum fill is to frosted line on tube and one GOLD SST minimum 3.0 mL.

For testing sent to Edmonton:
Two 2.7mL LT blue citrate tube, one GOLD SST minimum 1.0 mL, and another GOLD SST minimum 3.0 mL.

For pediatric and neonate testing contact Special Coagulation Laboratory

Specimen Collection Requirements:

Lt Blue Citrate: It is important to fill tube to maximum draw.
Mix well immediately after collection by inverting tube 3 to 4 times.
If venous access is not available, sample can be drawn from a line but 6x the volume of the line or 5 mL (whichever is less) must be removed prior to collection.
When using a winged blood collection set (butterfly needle), a discard tube must be used to remove the air in the tubing, if only a citrate tube is drawn or is the first tube drawn.

Test Resources:

Antiphospholipid Syndrome Investigation Requisition

Stability and Storage:

Room temperature – 3.5 hours

Processing:

For Gold SST:

For testing sent to Calgary:	For testing sent to Edmonton:
Centrifuge Gold SST tube	Referred in specimens must be sent as frozen serum Two 0.5 mL aliquots AND two 1.5 mL aliquots in plastic transport tubes Label aliquots as “serum”

For LT BLUE CITRATE specimens:

For testing sent to Calgary:	For testing sent to Edmonton:
For LT BLUE CITRATE specimens that will be received beyond stability: Centrifuge to obtain platelet poor plasma (platelet count <10 x 10*9/L) Aliquot plasma as per Special Coagulation Specimen Aliquoting	Centrifuge to obtain platelet poor plasma (platelet count <10 x 10*9/L) Aliquot plasma into 2 plastic aliquot tubes and freeze. Each aliquot must be at least 1.0 mL Aliquot as per Special Coagulation Aliquoting

Transportation:

Room temperature – uncentrifuged LT BLUE CITRATE and gold SST
Frozen (dry ice if available)

Routine Turn Around Time:

Calgary:
For stat testing contact Special Coagulation Laboratory
After hours contact the Hematopathologist on call.
Edmonton:
For stat testing MUST contact the Hematopathologist on call.
Test performed within 2 weeks of receipt of sample.

Testing Schedule:

Batched

Reference Interval:

See Laboratory Report.

Testing Area:

Anti-Beta-2 Glycoprotein	Calgary	Immunochemistry
Anti-Cardiolipin Antibody	Calgary	Immunochemistry
Anti-Cardiolipin Antibody	Edmonton	Special Coagulation
Lupus Anticoagulant	Calgary and Edmonton	Special Coagulation

Performing Site:

Calgary	APL Diagnostic and Scientific Centre
Edmonton	University of Alberta Hospital

Contact Comments:

1-877-868-6848

Last Updated On:

Wednesday, October 25, 2023