--- Query Results ---

  
Program:Genetics and Genomics
 
Test Name:Carnitine - Total and Free, urine (G&G South ACH)
Test Code:CARN-U
Performing Site: Alberta Children's Hospital
Performing Dept:Biochemical Genetics
 
Availability:Test performed on a biweekly basis.
TAT:30 days
 
Preferred Tube/Container:Random specimen: plain urine container
 
Specimen Requirements:Collect 5-10 mL random urine sample.
 
Min. Sample Required:1.0 mL urine
 
Specimen Processing:1.  If specimen is transferred to secondary aliquot tube or container, write "urine" on label.
2.  Freeze.
 
Specimen Handling:Transport frozen samples on ice. Samples must not thaw during transport.
 
Additional Information:Samples will be rejected if:
  • Aliquot received thawed
  • Sample type not written on aliquot tube label
  •  
    Requisition/Form:Submit request using Biochemical Genetics Laboratory Requisition.
    Please ensure all required information, including patient's clinical history/indication, is provided on the requisition.
     
    Order Restrictions:This test is considered specialty testing; this test will be cancelled if sufficient clinical justification is not provided.
    This test should be ordered in conjunction with Carnitine, Total and Free - Blood (ACH), or following findings of abnormal plasma/serum levels, as the interpretation of these findings are dependent on the patient's plasma or serum levels.
     
    Indications:This test is for the further investigation of a patient with clear evidence of a systemic carnitine deficiency based on low plasma/serum total and free carnitine levels, to determine whether the cause of their deficiency is due to specific renal loss (i.e., carnitine uptake defect) or generalized renal insufficiency. Differentiating between primary and secondary cause can be evaluated by calculating fractional carnitine excretion.
    Method:Total and free carnitine levels are measured with and without acid hydrolysis, and the acylcarnitine fraction calculated, using Liquid Chromatography, Electrospray-Ionization Tandem Mass Spectrometry (LC-MS/MS) with a deuterium-labelled internal standard.
    Method Details:Determination of urine total and free carnitine levels enables the confirmation of a systemic carnitine deficiency due to renal loss, and can be influenced by current nutritional status and the application of carnitine supplementation therapy. Therefore, such information should be provided on the requisition.
     
    Reference Interval:
    Total Carnitine20.4 - 46.6mmol/mol creatinine
    Free Carnitine  8.7 - 24.2mmol/mol creatinine
    Acyl/Free Carnitine   0.7 - 3.4ratio
     
    Comments:Contact ACH Biochemical Genetics Laboratory (403-955-7379) for priority turn-around.
     
     
    Last Updated On:Thursday, August 31, 2017
    Date of Last Review:Jan 2 2018 12:00AM