--- Query Results ---

  
Program:Genetics and Genomics
 
Test Name:Hydroxybutyrate, beta (G&G South ACH)
Test Code:3HB
Performing Site: Alberta Children's Hospital
Performing Dept:Biochemical Genetics
 
Availability:Test performed on a weekly basis.
TAT:7 days
 
Preferred Tube/Container:Sodium heparin (dark green - NOT PST)
Alternate:Lithium heparin (dark green - NOT PST)
Sodium fluoride (grey)
 
Specimen Requirements:1.  Collect 1 x 4.0 mL sodium heparin tube (or alternate tube type).
2.  Collection after an 8 to 15 hour fast preferred (but not mandatory).
3.  Indicate length of fast on the requisition (with collection time).
4.  If patient is on ketogenic diet, indicate whether sample is fed or fasted.
5.  Physician test requests from known metabolic patients (non-fasting) are also acceptable.
 
Min. Sample Required:0.25 mL plasma
 
Specimen Processing:At ACH only
The primary heparinized tube can be put on ice (ensure not to freeze sample) or refrigerated for routine pick-ups between 8:30 am – 2:30 pm, Monday through Friday.

For all other collections
1. Centrifuge.
2. Transfer plasma or serum to aliquot tube.
3. Write "NaHep", "LiHep" or "Red Top" on the aliquot tube label.
4. Freeze.
 
Specimen Handling:Transport frozen samples on ice. Samples must not thaw during transport.
 
Additional Information:Samples will be rejected if:
  • Primary tube not refrigerated
  • Aliquot tube received thawed
  • Tube type not written on aliquot tube label
  •  
    Requisition/Form:Submit request using Biochemical Genetics Laboratory Requisition.
    Please ensure all required information, including patient's clinical history/indication, is provided on the requisition.
     
    Order Restrictions:None
     
    Indications:Ketosis is a common feature in acutely ill patients and in some subjects can result in severe life threatening metabolic acidosis. The presence and degree of ketosis can be determined by measuring blood levels of 3-hydroxybutyrate (or D-3-hydroxybutyrate).

    This test is for evaluating patients suspected of having an inborn error of metabolism affecting energy production (e.g., hepatic glycogen storage disease) or utilization (e.g., fatty acid oxidation disorders). This test can also be used to monitor adherence of patients treated with the ketogenic diet. This test may also help in the evaluation of diabetes, as well as other conditions leading to or resulting from carbohydrate deprivation, reduced carbohydrate utilization, ketoacidosis, alkalosis or altered cellular redox state.
    Method:Beta-hydroxybutyrate is detected spectrophotometrically using a commercially available kit
    Method Details:Metabolite reported:
  • D-3-hydroxybutyrate
  •  
    Reference Interval:Provided on test report, based on patient age and prandial state.
     
    Comments:Contact ACH Biochemical Genetics Laboratory (403-955-7379) for priority turn-around.
     
     
    Last Updated On:Monday, March 26, 2018
    Date of Last Review:Jan 2 2018 12:00AM