--- Query Results ---

  
Program:Genetics and Genomics
 
Test Name:t(15;17) PML/RARA
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
 
Availability:Test performed Monday to Friday
TAT:Variable - see Comments section at bottom of page
 
Preferred Tube/Container:See Specimen Requirements
 
Specimen Requirements:For Blood Samples:Unopened dedicated tubes are required for Molecular Pathology testing
1. Any peripheral blood requests will require a CBC and differential to be ordered and processed as per local handling practices.
2. Collect 2 x 4 mL EDTA (lavender) tubes. One tube for CBC/differential and one tube for Molecular Pathology.
3. Collect 1 x PAXgene Blood RNA for Molecular Pathology.
4. Ensure to follow the specific specimen collection requirements.
Using a butterfly needle and holding the tube below the arm to prevent backflow of contents into the patient, first collect 2 x 4 mL EDTA tubes, then 2.5 mL blood into the PAXgene Blood RNA tube.
Collection must be in the correct order of draw to ensure accurate white count determination.

For Bone Marrow Samples:Unopened dedicated tubes are required for Molecular Pathology testing
1. Collect 1 x 4mL EDTA (lavender) tube.
2. Collect 1 x PAXgene Bone Marrow RNA tube.
3. Ensure to follow the specific specimen collection requirements.
Place 2 mL of bone marrow into the EDTA tube and not more than 2 mL of bone marrow into the PAXgene Bone Marrow RNA tube. Order of draw is not important.
 
Specimen Processing:Immediately following collection, place all tubes ON ICE.
Do not spin.
Keep samples refrigerated until ready for transport.
 
Specimen Handling:Transport samples to Molecular Pathology as soon as possible, on ice or cold pack.
Molecular Pathology must receive the sample within 24 hours of collection.
If transport to Molecular Pathology is anticipated to be greater than 24 hours, contact Molecular Pathology at 780-407-6648.
 
Additional Information:PAXgene collection tubes are available upon request. Submit an order form using one of the links provided: Client Supply Order Edmonton Zone or Client Supply Order Provincial Form
 
Requisition/Form:All requests MUST be submitted on a Molecular Pathology Requisition.
Please provide any pertinent clinical history on the requisition.
 
Indications:For the investigation of myeloproliferative neoplasms and/or acute leukemias.
Gene:PML/RARA
 
Method:Real-time PCR
Method Details:PML/RARA Detection Assay
RNA extraction is performed on the QIAsymphony. cDNA synthesis and amplification of t(15;17) gene transcripts is performed in two steps by RT qPCR with ABL1 as the reference gene. Fluorescence detection is by the Quant Studio real time PCR platform (Life Technologies). Appropriate positive and negative controls are used.

The limit of detection (LOD) of this assay is a 5.0 log reduction from the mean of pooled positive diagnostic samples.

PML/RARA Quantitation Assay
RNA extraction is performed on the QIAsymphony. cDNA synthesis and quantitation of t(15:17) gene transcripts is performed in two steps by RT-qPCR with ABL1 as the reference gene. Fluorescence detection is by the Quant Studio real time PCR platform (Life Technologies). Appropriate positive and negative controls are used.

The results are expressed as percentage residual disease and a calculated log reduction (LR). The residual disease is the percentage of NCN of the monitor sample to the NCN of the initial diagnostic sample. The LR is the log reduction in the normalized copy number (NCN) of PML-RARA transcript from the initial diagnostic sample. The NCN is the ratio of PML-RARA transcripts to ABL1 transcripts.

The limit of detection (LOD) of this assay is a 5.0-log reduction from the mean of pooled positive diagnostic samples, which corresponds to 0.001% residual disease.
 
Comments:Detection Assay Turn Around Time - RUSH 1 day, routine 3 days
Quantitative Assay Turn Around Time - 1 week
 
 
Last Updated On:Wednesday, June 21, 2017
Date of Last Review:Jan 2 2018 12:00AM