--- Query Results ---

Program:Genetics and Genomics
Test Name:Factor V Leiden Mutation
Test Code:APCGN
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
Availability:Test performed on second Wednesday of each month.
TAT:1 month
Preferred Tube/Container:See Specimen Requirements
Specimen Requirements:Unopened dedicated tubes are required for Molecular Pathology testing.
1. Collect 1 x 4 mL EDTA (lavender) tube.
Specimen Processing:Do not spin.
Do not freeze.
Keep sample refrigerated until ready for transport.
Specimen Handling:Must be received in Molecular Pathology laboratory within 3 days of collection.
Additional Information:Factor V Leiden mutation requests must be ordered in conjunction with APC Resistance, Plasma
1. Refer to Activated Protein C Resistance, plasma (APCE). for details of collection.
2. Submit request for both tests using the Hematology Specialty Requisition.
Requisition/Form:All requests MUST be submitted on the Hematology Specialty Requisition .
Please provide any pertinent clinical history on the requisition.
Indications:Investigation of thrombophilia.
Method:Real-time PCR
Method Details:After DNA isolation from peripheral blood sample, polymerase chain reaction is performed using the LightCycler 2.0 instrument and the Factor V Leiden kit purchased from F.Hoffman-La Roche Ltd. (Basel, Switzerland).

The assay utilizes fluorogenic target-specific hybridization for genotyping of the amplified Factor V DNA and allows the detection of a single point mutation (G to A at position 1691) of the human Factor V gene. Data analysis is performed using the software purchased from the company.
Last Updated On:Tuesday, June 27, 2017
Date of Last Review:Jan 2 2018 12:00AM