--- Query Results ---

  
Program:Genetics and Genomics
 
Test Name:Prothrombin G20210A Mutation
Test Code:PROM
 
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
 
Availability:Test performed on Thursday each week.
TAT:2 weeks
 
Preferred Tube/Container:See Specimen Requirements
 
Specimen Requirements:Unopened dedicated tubes are required for Molecular Pathology testing.
1. Collect 1 x 4 mL EDTA (lavender) tube.
 
Specimen Processing:Do not spin.
Do not freeze.
Keep sample refrigerated until ready for transport.
 
Specimen Handling:Must be received in Molecular Pathology laboratory within 3 days of collection.
 
Requisition/Form:All requests MUST be submitted on a Molecular Pathology Requisition.
Please provide any pertinent clinical history on the requisition.
 
Indications:Investigation of thrombophilia.
Method:Real-time PCR
Method Details:After DNA isolation from peripheral blood sample, polymerase chain reaction is performed using the LightCycler 2.0 instrument and the Factor II G20210A kit purchased from F.Hoffman-La Roche Ltd. (Basel, Switzerland).

The assay utilizes fluorogenic target-specific hybridization for genotyping of the amplified Factor II DNA and allows the detection of a single point mutation (G to A at position 20210) of the human Factor II gene. Data analysis is performed using the software purchased from the company.
 
 
Last Updated On:Tuesday, June 27, 2017
Date of Last Review:Jan 2 2018 12:00AM