--- Query Results ---

Program:Genetics and Genomics
Test Name:Microsatellite Instability
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
Availability:Test performed every second week.
TAT:1 month
Preferred Tube/Container:See Specimen Requirements
Specimen Requirements: For Paraffin-Embedded Tissue:
1. Representative formalin-fixed, paraffin-embedded tissue block(s) of tumor and normal (uninvolved) tissue is preferred. A 1 x 4 mL EDTA blood sample may also be used as a normal specimen.
2. Alternatively, one slide stained with Hematoxylin & Eosin as well as five 10-micron non-baked unstained slides can be submitted.
3. Please provide a copy of the corresponding pathology report.
Additional Information:Test requires comparison between tumor and normal specimens. Test will not be performed if a normal tissue or blood sample is not submitted.
Requisition/Form:All requests MUST be submitted on a Molecular Pathology Requisition.
Please provide any pertinent clinical history on the requisition.
Indications:For the investigation of colonic, ovarian and uterine adenocarcinomas.
Method:PCR followed by capillary electrophoresis.
Method Details:The assay involves multiplex PCR of five microsatellite (MS) loci for both normal and tumor tissue. The MS loci tested include mononucleotide repeats BAT25, BAT26, BAT40, CAT25 and NR21.

GUIDE TO TEST RESULT: Comparative evaluation of normal and tumor peak patterns may reveal shifts in one or more MS sequences amplified in the tumor, representing microsatellite instability (MSI). Tumor microsatellite instability is classified as follows: high frequency instability (MSI-H) when two or more sequences demonstrate instability, low frequency instability (MSI-L) if one of the sequences demonstrate instability, and microsatellite stable (MSS) when no shifts are evident.
Last Updated On:Wednesday, June 14, 2017
Date of Last Review:Jan 2 2018 12:00AM