--- Query Results ---

  
Program:Genetics and Genomics
 
Test Name:IgH Gene Rearrangement
Alternate Test Name:B Cell Gene Rearrangement
 
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
 
Availability:Test set-up on Monday and Thursday (excluding statutory holidays).
TAT:1 week
 
Preferred Tube/Container:See Specimen Requirements
 
Specimen Requirements:For Blood Samples: Unopened dedicated tubes are required for Molecular Pathology testing.
1. Any peripheral blood requests will require a CBC and differential to be ordered and processed as per local handling practices.
2. Collect 2 x 4 mL EDTA (lavender) tubes. One tube for CBC/differential and one tube for Molecular Pathology.

For Bone Marrow Samples: Unopened dedicated tubes are required for Molecular Pathology testing.
1. Collect 1 x 4 mL EDTA (lavender) tube.
2. Minimum 2 mL of bone marrow required.

For Paraffin-Embedded Tissue:
1. A formalin-fixed, paraffin-embedded tissue block is preferred.
2. Alternatively, one slide stained with Hematoxylin & Eosin and five 10-micron non-baked, unstained slides can be submitted.
3. Please provide a copy of the corresponding pathology report.
 
Specimen Processing:Do not spin.
Do not freeze.
Keep samples refrigerated until ready to transport.
 
Specimen Handling:Whole blood samples must be received in Molecular Pathology within 3 days of collection.
Transport samples at ambient temperature.
 
Requisition/Form:All requests MUST be submitted on a Molecular Pathology Requisition.
Please provide any pertinent clinical history on the requisition.
 
Order Restrictions:Test requests are restricted to hematopathologists and lymphoma pathologists.
 
Indications:For the investigation of lymphoproliferative disorders.
Gene:IgH
 
Method:PCR followed by capillary electrophoresis.
Method Details:DNA is extracted using the QIAsymphony DSP DNA Mini Kit from Qiagen (Valencia, CA.). A commercially available kit is purchased from InVivoScribe Technologies (San Diego, California) and utilized in this assay. This assay has been validated internally using >30 B-cell lymphomas or benign lymphoid tissues for which the diagnoses were confirmed histologically and immunophenotypically.

PCR is performed using Invitrogen Platinum High Fidelity Taq with fluorescence-labelled primers in three different mixes. The PCR products are subjected to capillary electrophoresis on an Applied Biosystems 3130/3500XL Genetic Analyzer using fragment analysis GeneMapper software. The integrity of the DNA is assessed using a Specimen Control Size Ladder detecting four different fragment sizes. Appropriate polyclonal and monoclonal controls (B-cell lymphoma cell lines) are used. A no template control is also included.

This assay does not detect 100% clonal cell populations and cannot detect less than 5 positive cells/95 normal cells.

The clinical sensitivity of this assay is determined to be 75% and clinical specificity is 100%.
 
 
Last Updated On:Friday, June 23, 2017
Date of Last Review:Jan 2 2018 12:00AM