--- Query Results ---

  
Program:Genetics and Genomics
 
Test Name:MYC 8q24 by FISH
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
 
Availability:Test set-up on Monday and Wednesday (excluding statutory holidays).
TAT:1 week for routine or 3 days for RUSH
 
Preferred Tube/Container:See Specimen Requirements
 
Specimen Requirements: For Paraffin-Embedded Tissue:
1. A formalin-fixed, paraffin-embedded tissue block is preferred.
2. Alternatively, one slide stained with Hematoxylin & Eosin as well as six 4-micron baked (2 hours at 60°C) unstained charged slides can be submitted.
3. Please provide a copy of the corresponding pathology report.
 
Requisition/Form:All requests MUST be submitted on a Molecular Pathology Requisition.
Please provide any pertinent clinical history on the requisition.
 
Indications:For the investigation of:
  • Burkitt’s lymphoma
  • Diffuse large B-cell lymphoma
  • Gene:MYC
     
    Method:Fluorescence In Situ Hybridization
    Method Details:Slides are cut from the paraffin block and are deparaffinized in xylene and dehydrated in ethanol. Specimen pretreatment and fluorescence in situ hybridization (FISH) is performed using Vysis® (Abbott Molecular Inc.). Pretreatment kit and appropriate probes as described below.

    LSI MYC Dual Color Break-apart rearrangement probe hybridizes to the band region 8q24. In normal cells, the two probes (green and orange) are fused or close together. In abnormal cells, with a break of 8q24, the two probes separate. This probe detects the translocation: t(8;14)IGH-MYC, which is most characteristic of Burkitt’s lymphoma/leukemia. This probe should also detect two variants t(2;8)IGK-MYC and t(8;22)IGL-MYC. About 5 to 10% of diffuse large B-cell lymphoma (DLBCL) patients also have MYC region rearrangements, and detection of these rearrangements has been associated with a poor prognosis. It has been suggested that FISH analysis for MYC rearrangements should be performed on all DLBCL patients. This lab’s normal cut off is established at <10% of the cells showing an abnormal signal pattern.

    All appropriate negative and positive controls were used. An average of 100 interphase nuclei were examined independently by two observers using an Olympus Provis Fluorescence microscope system on 1000X magnification.
     
     
    Last Updated On:Wednesday, June 14, 2017
    Date of Last Review:Jan 2 2018 12:00AM