--- Query Results ---

Program:Genetics and Genomics
Test Name:KRAS Mutation Analysis
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
Availability:Test set-up on Monday and Wednesday (excluding statutory holidays).
TAT:2 weeks
Preferred Tube/Container:See Specimen Requirements
Specimen Requirements: For Paraffin-Embedded Tissue:
1. A formalin-fixed, paraffin-embedded tissue block is preferred.
2. Alternatively, one slide stained with Hematoxylin & Eosin as well as five 10-micron non-baked unstained slides can be submitted.
3. Please provide a copy of the corresponding pathology report.
Requisition/Form:All requests MUST be submitted on a Molecular Pathology Requisition.
Please provide any pertinent clinical history on the requisition.
Indications:For investigation of colon cancer.
Method:PCR followed by capillary electrophoresis.
Method Details:DNA extraction from FFPE samples is performed using the QIAGEN QIAsymphony DSP DNA mini kit. Analysis for BRAF, KRAS, NRAS mutations is performed after PCR amplification using a single base extension technique (SNaPshot multiplex kit by Life Technologies) and capillary electrophoresis on the 3130 Genetic Analyzer. This multiplexed assay is capable of detecting mutations at BRAF codon 600 and KRAS/NRAS codons 12, 13, 61, 117, and 146.
The estimated sensitivity of this assay is 10%.
Last Updated On:Tuesday, June 13, 2017
Date of Last Review:Jan 2 2018 12:00AM