--- Query Results ---

  
Program:Genetics and Genomics
 
Test Name:Calreticulin
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
 
Availability:Test set-up Monday, bi-weekly.
TAT:2 weeks
 
Preferred Tube/Container:See Specimen Requirements
 
Specimen Requirements:For Blood Samples:Unopened dedicated tubes are required for Molecular Pathology testing.
1. Any peripheral blood requests will require a CBC and differential to be ordered and processed as per local handling practices.
2. Collect 2 x 4 mL EDTA (lavender) tubes. One tube for CBC/differential and one tube for Molecular Pathology.

For Bone Marrow Samples:Unopened dedicated tubes are required for Molecular Pathology testing.
1. Collect a minimum of 2 mL in 1 x 4 mL EDTA (lavender) tube.
 
Specimen Processing:Do not spin.
Do not freeze.
Keep samples refrigerated until ready for transport.
 
Specimen Handling:Samples must be received in the Molecular Pathology within 3 days of collection.
 
Requisition/Form:All requests MUST be submitted on a Molecular Pathology Requisition.
Please provide any pertinent clinical history on the requisition.
 
Indications:For the investigation of myeloproliferative neoplasms.
Gene:CalR
 
Method:PCR followed by capillary electrophoresis
Method Details:After DNA extraction, PCR amplification for CalR gene is performed in a single tube using fluorescently labeled primers. Amplification products are subjected to fragment analysis by capillary electrophoresis using ABI 3130 genetic analyzer. Results were analyzed by Genemapper v3.2 software.

The analytical sensitivity of this assay is 5%.
 
 
Last Updated On:Wednesday, June 21, 2017
Date of Last Review:Jan 2 2018 12:00AM