--- Query Results ---

  
Program:Genetics and Genomics
 
Test Name:EGFR Mutation Analysis
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
 
Availability:Test set-up on Friday (excluding statutory holidays).
TAT:2 weeks
 
Preferred Tube/Container:See Specimen Requirements
 
Specimen Requirements: For Paraffin-Embedded Tissue:
1. A formalin-fixed, paraffin-embedded tissue block is preferred.
2. Alternatively, one slide stained with Hematoxylin & Eosin as well as five 10-micron non-baked unstained slides can be submitted.
3. Please provide a copy of the corresponding pathology report.
 
Requisition/Form:All requests MUST be submitted on a Molecular Pathology Requisition.
Please provide any pertinent clinical history on the requisition.
 
Indications:For the investigation of non-small cell lung cancers (NSCLC).
Gene:EGFR
 
Method:Real-time PCR
Method Details:DNA extraction from FFPE is performed using the QIAsymphony DSP DNA Mini Kit from Qiagen (Valencia, CA.). The EGFR Mutation Assay is a Real-Time PCR assay performed using the QIAGEN EGFR RGQ PCR kit run on the Life Technologies QuantStudio instrument. This assay is capable of detecting Exon 19 deletions, L858R, L861Q, G719X (identifies but does not differentiate G719A/S/C), S768I, Exon 20 insertions, and T790M mutations.

The estimated sensitivity of this assay is 1-3%.
 
 
Last Updated On:Friday, June 9, 2017
Date of Last Review:Jan 2 2018 12:00AM