--- Query Results ---

  
Program:Genetics and Genomics
 
Test Name:FLT3
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
 
Availability:Test set-up twice a week.
TAT:3 business days (5 calendar days) for new AML
 
Preferred Tube/Container:See Specimen Requirements
 
Specimen Requirements:For Blood Samples:Unopened dedicated tubes are required for Molecular Pathology testing.
1. Any peripheral blood requests will require a CBC and differential to be ordered and processed as per local handling practices.
2. Collect 2 x 4 mL EDTA (lavender) tubes. One tube for CBC/differential and one tube for Molecular Pathology

For Bone Marrow Samples:Unopened dedicated tubes are required for Molecular Pathology testing.
1. Collect a minimum of 2 mL in 1 x 4 mL EDTA (lavender) tube.
 
Specimen Processing:Do not spin.
Do not freeze.
Keep samples refrigerated until ready for transport.
 
Specimen Handling:Whole blood samples must be received in the Molecular Pathology Laboratory within 3 days of collection.
 
Additional Information:CEBPA is a reflexed test that is only performed when the assay results for FLT3 / NPM1 are negative and the patient's karyotype is normal.
 
Requisition/Form:All requests MUST be submitted on a Molecular Pathology Requisition.
Please provide any pertinent clinical history on the requisition.
 
Indications:For the investigation of acute myelogenous leukemias (AML).
Gene:FLT3
 
Method:PCR followed by capillary electrophoresis.
Method Details:After DNA extraction, PCR amplification is performed in two separate tubes for FLT3 and NPM1 using fluorescence labeled primers. Amplification products are subjected to fragment analysis by capillary electrophoresis using ABI 3130 genetic analyzer. Results are analyzed by Genemapper v3.2 software.

The sensitivity of these assays is 5%.
 
 
Last Updated On:Thursday, May 2, 2019
Date of Last Review:Jan 2 2018 12:00AM