--- Query Results ---

  
Program:Genetics and Genomics
 
Test Name:JAK2 Exon 12 Mutation
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
 
Availability:Test set-up on Wednesday.
TAT:2 weeks
 
Preferred Tube/Container:See Specimen Requirements
 
Specimen Requirements:For Blood Samples:Unopened dedicated tubes are required for Molecular Pathology testing.
1. Any peripheral blood requests will require a CBC and differential to be ordered and processed as per local handling practices.
2. Collect 2 x 4 mL EDTA (lavender) tubes. One tube for CBC/differential and one tube for Molecular Pathology.

For Bone Marrow Samples:Unopened dedicated tubes are required for Molecular Pathology testing.
1. Collect a minimum of 2 mL in 1 x 4 mL EDTA (lavender) tube.
 
Specimen Processing:Do not spin.
Do not freeze.
Keep samples refrigerated until ready for transport.
 
Specimen Handling:Samples must be received in the Molecular Pathology Laboratory within 3 days of collection.
 
Requisition/Form:All requests MUST be submitted on a Molecular Pathology Requisition.
Please provide any pertinent clinical history on the requisition.
 
Indications:For the investigation of myeloproliferative neoplasms.
Gene:JAK2
 
Method:PCR and sequence analysis
Method Details:DNA is extracted using the QIAsymphony DSP DNA Mini Kit from Qiagen (Valencia, CA.). PCR amplification is performed using M13 tailed primers. Bidirectional sequencing of the product is performed using the BigDye Direct Cycle Sequencing kit (Life Technologies) and run on the Applied Biosystems 3500xL Genetic Analyzer. The obtained DNA sequence is then analyzed using ABI SeqScanner® and SeqPilot® software programs and compared to the published sequences in the National Center for Biotechnology Information (NCBI) database at www.ncbi.nlm.nih.gov. (GenBank).

Analytical sensitivity is 20%.
 
 
Last Updated On:Wednesday, June 21, 2017
Date of Last Review:Jan 2 2018 12:00AM