--- Query Results ---

Program:Genetics and Genomics
Test Name:KIT for Melanoma / GIST
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
Availability:Test set-up on Wednesday.
TAT:2 weeks
Preferred Tube/Container:See Specimen Requirements
Specimen Requirements: For Paraffin-Embedded Tissue:
1. A formalin-fixed, paraffin-embedded tissue block is preferred.
2. Alternatively, one slide stained with Hematoxylin & Eosin as well as five 10-micron non-baked unstained slides can be submitted.
3. Please provide a copy of the corresponding pathology report.
Requisition/Form:All requests MUST be submitted on a Molecular Pathology Requisition.
Please provide any pertinent clinical history on the requisition.
Indications:1. For the investigation of melanoma.
2. When ordered in conjunction with PDGFRA used for the investigation of Gastrointestinal Stromal Tumours (GIST).
Method:PCR and sequence analysis
Method Details:DNA is extracted using the QIAsymphony DSP DNA Mini Kit from Qiagen (Valencia, CA.). The exact area of tumorous tissue used in the assay is identified by histological examination and micro dissected from unstained slides.

PCR amplification is performed using M13 tagged primers. Bidirectional sequencing of the product was performed using the Big dye terminator kit (Life Technologies) and run on the Applied Biosystems 3130 Genetic Analyzer. The obtained DNA sequence was then analyzed using ABI Seqscape® software and compared to the published sequences in the National Center for Biotechnology Information (NCBI) database at www.ncbi.nlm.nih.gov. (GenBank).

Analytical sensitivity is 20%
Last Updated On:Friday, June 9, 2017
Date of Last Review:Jan 2 2018 12:00AM