--- Query Results ---

Program:Genetics and Genomics
Test Name:KIT for Mastocytosis
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
Availability:Test set-up Monday, bi-weekly.
TAT:2 weeks
Preferred Tube/Container:See Specimen Requirements
Specimen Requirements:For Blood Samples:Unopened dedicated tubes are required for Molecular Pathology testing.
1. Any peripheral blood requests will require a CBC and differential to be ordered and processed as per local handling practices.
2. Collect 2 x 4 mL EDTA (lavender) tubes. One tube for CBC/differential and one tube for Molecular Pathology.

For Bone Marrow Samples:Unopened dedicated tubes are required for Molecular Pathology testing.
1. Collect a minimum of 2 mL in 1 x 4 mL EDTA (lavender) tube.

For Paraffin-Embedded Tissue:
1. A formalin-fixed, paraffin-embedded tissue block is preferred.
2. Alternatively, one slide stained with Hematoxylin & Eosin as well as five 10-micron non-baked unstained slides can be submitted.
3. Please provide a copy of the corresponding pathology report.
Specimen Processing:Do not spin.
Do not freeze.
Keep samples refrigerated until ready for transport.
Specimen Handling:Samples must be received in the Molecular Pathology within 3 days of collection.
Requisition/Form:All requests MUST be submitted on a Molecular Pathology Requisition.
Please provide any pertinent clinical history on the requisition.
Indications:For the investigation of systemic mastocytosis.
Method:PCR and capillary electrophoresis
Method Details:DNA is extracted on the QIAsymphony instrument. Allele specific PCR amplification is performed in two separate tubes for mutant and wildtype KIT using fluorescently labeled primers. Amplification products are subjected to fragment analysis by capillary electrophoresis using ABI 3130/3500 genetic analyzer. Results are analyzed by Genemapper v3.2 software.

The sensitivity of this assay is 1%..
Last Updated On:Monday, October 7, 2019
Date of Last Review:Jan 2 2018 12:00AM