--- Query Results ---

Program:Genetics and Genomics
Test Name:MPL Mutation
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
Availability:Test performed every second week.
TAT:2 weeks
Preferred Tube/Container:See Specimen Requirements
Specimen Requirements:For Blood Samples:Unopened dedicated tubes are required for Molecular Pathology testing.
1. Any peripheral blood requests will require a CBC and differential to be ordered and processed as per local handling practices.
2. Collect 2 x 4 mL EDTA (lavender) tubes. One tube for CBC/differential and one tube for Molecular Pathology

For Bone Marrow Samples:Unopened dedicated tubes are required for Molecular Pathology testing.
1. Collect a minimum of 2 mL in 1 x 4 mL EDTA (lavender) tube.
Specimen Processing:Do not spin.
Do not freeze.
Keep samples refrigerated until ready for transport.
Specimen Handling:Whole blood samples must be received in the Molecular Pathology Laboratory within 3 days of collection.
Requisition/Form:All requests MUST be submitted on a Molecular Pathology Requisition.
Please provide any pertinent clinical history on the requisition.
Indications:For the investigation of myeloproliferative neoplasms.
Method:PCR followed by capillary electrophoresis.
Method Details:After DNA extraction, multiplex allele specific PCR amplification for MPL gene exon 10 mutations is performed in two tubes using fluorescently labeled primers to detect the four most common mutations. Amplification products are subjected to fragment analysis by capillary electrophoresis using ABI 3130/3500xL genetic analyzer. Results are analyzed by Genemapper v3.2 software.

The analytic sensitivity of this assay is 2%..
Last Updated On:Tuesday, June 27, 2017
Date of Last Review:Jan 2 2018 12:00AM