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Program:Genetics and Genomics
Test Name:Synovial Sarcoma by FISH
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
Availability:Test set-up on Monday and Wednesday (excluding statutory holidays).
TAT:2 weeks
Preferred Tube/Container:See Specimen Requirements
Specimen Requirements: For Paraffin-Embedded Tissue:
1. A formalin-fixed, paraffin-embedded tissue block is preferred.
2. Alternatively, one slide stained with Hematoxylin & Eosin as well as six 4-micron baked (2 hours at 60C) unstained charged slides can be submitted.
3. Please provide a copy of the corresponding pathology report.
Requisition/Form:All requests MUST be submitted on a Molecular Pathology Requisition.
Please provide any pertinent clinical history on the requisition.
Indications:For investigation of Synovial Sarcoma.
Method:Fluorescence In Situ Hybridization
Method Details:Slides are cut from paraffin blocks and are deparaffinized in xylene and dehydrated in ethanol. Specimen pretreatment and fluorescence in situ hybridization (FISH) are performed using Vysis® (Abbott Molecular Inc.) pretreatment kit and appropriate probes as described below.

The locus specific identifier (LSI) SS18 (SYT) dual colour break apart gene rearrangement probe is a mixture of two FISH DNA probes that hybridize to opposite sides of the SS18 gene. Synovial sarcoma is characterized by a
t(X;18)(p11.2;q11.2) which is present in virtually all examples. This translocation fuses SYT on chromosome 18 with either SSX1 (66%) or SSX2 (33%), both located on chromosome Xp11. More rarely, SYT is adjoined to SSX4 also located on chromosome Xp11. The majority of breakpoints for t(X;18)(p11.2;q11.2) is not found at random and arises exclusively in Synovial sarcoma. In normal cells, the two probes (green and orange) are fused or close together. Normal nuclei contain two red/green (yellow) fusion signals (2F). In abnormal cells, with
t(X;18)(p11.2;q11.2), having a breakpoint within the hybridization targets of the probes exhibits one yellow, one red and one green signal (FRG).

This lab’s normal cut off is established at less than or equal to 10% of the cells showing an abnormal signal pattern. All appropriate negative and positive controls are used.

An average of 100 interphase nuclei are examined independently by two observers using an Olympus Provis Fluorescence microscope system on 1000X magnification.
Last Updated On:Tuesday, June 27, 2017
Date of Last Review:Jan 2 2018 12:00AM