--- Query Results ---

  
Program:Genetics and Genomics
 
Test Name:Prosigna for Breast Cancer
Performing Site: University of Alberta Hospital
Performing Dept:Molecular Pathology
 
Availability:Test set-up on Monday and Wednesday (excluding statutory holidays).
TAT:1 week
 
Preferred Tube/Container:See Specimen Requirements
 
Specimen Requirements:For Paraffin-Embedded Tissue:
Cut the block in the following order:
1.  1 x 4-micron Hematoxylin & Eosin (H&E) stained slide
2.  6 x 10-micron unbaked unstained charged slides
3.  1 x 4-micron Hematoxylin & Eosin (H&E) stained slide
 
Specimen Processing:The breast biomarker pathologist must circle the tumor area in the first H&E slide.
In addition, the breast biomarker pathologist needs to ensure that the tumor cellularity is >10% and the surface area is > 4 mm2.

Please provide a copy of the corresponding pathology report.
 
Additional Information:Please note: Aptex or Apex slides are not acceptable.
Electrostatically charged slides must be used.
 
Requisition/Form:All requests MUST be submitted on a Molecular Pathology Requisition.
Please provide any pertinent clinical history on the requisition.
 
Indications:To determine breast cancer recurrence risk in women with hormone receptor positive, HER2 negative, node negative breast cancers with equivocal clinico-pathologic characteristics. Test restricted to breast oncologists and pathologists.
Method:Gene expression profile
Method Details:Prosigna is a Health Canada approved test that uses a 50 gene expression profile, tumour size and nodal status to identify intrinsic subtype, report a Risk of Recurrence Score (ROR) and a probability of Distant Recurrence (DR). RNA is extracted from FFPE tissue, hybridized to the Prosigna CodeSet in the nCounter prep station and scanned by the nCounter Digital Analyzer.
 
 
Last Updated On:Friday, January 19, 2018
Date of Last Review:Jan 2 2018 12:00AM